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首页> 外文期刊>Molecular and Cellular Biology >Loss of chloroplast DNA methylation during dedifferentiation of Chlamydomonas reinhardi gametes.
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Loss of chloroplast DNA methylation during dedifferentiation of Chlamydomonas reinhardi gametes.

机译：莱茵衣藻配子去分化过程中叶绿体DNA甲基化的损失。

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In Chlamydomonas reinhardi the chloroplast DNA (ch;DNA) of mating type plus cells undergoes cyclical methylation and demethylation during the life cycle. Methylation occurs during gametogenesis, and fully differentiated gametes can be dedifferentiated back to vegetative cells which contain nonmethylated chlDNA by the addition of a nitrogen source for growth. We examined the dedifferentiation process and found that the mating ability of gametes was lost rapidly after the start of dedifferentiation at a time when the chlDNA was still methylated. The enzymatic activity of the 200-kilodalton DNA methyltransferase was lost at a rate consistent with the rate of dilution during cell division. Methylation of chlDNA decreased at a slower rate than was expected from cell division alone but was consistent with the continuing activity of the preexisting methyltransferase so long as it was present. These results support the hypothesis that demethylation of chlDNA occurs by dilution out of enzymatic methylating activity rather than by enzymatic demethylation.
机译：在莱茵衣藻中，交配型加细胞的叶绿体DNA（ch; DNA）在生命周期中经历了循环甲基化和去甲基化。甲基化发生在配子发生期间，并且可以通过添加氮源进行生长来使完全分化的配子去分化为包含非甲基化chlDNA的营养细胞。我们检查了去分化过程，发现当chlDNA仍被甲基化时，去分化开始后，配子的交配能力迅速丧失。 200-千达尔顿DNA甲基转移酶的酶活性丧失的速率与细胞分裂过程中的稀释速率一致。 chlDNA的甲基化下降速度低于单独细胞分裂所预期的下降速度，但与存在的甲基转移酶的持续活性一致，只要存在即可。这些结果支持这样的假说，即chlDNA的去甲基化是通过稀释而不是酶促甲基化活性而不是酶促去甲基化发生的。

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