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首页> 外文期刊>Molecular and Cellular Biology >Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.
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Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.

机译：酿酒酵母的HIS4基因的正调控相互作用。

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摘要

The role of cis- and trans-acting elements in the expression of HIS4 has been examined by using HIS4-lacZ fusions in which lacZ expression is dependent upon the HIS4 5' noncoding region. The cis-acting sequences involved in regulation were defined by studying the effects of the wild-type and various deletions and their revertants on regulation via the general control of amino acid biosynthesis. The role of trans-acting genes was analyzed by studying the regulation of the HIS4-lacZ fusions in strains carrying mutations in the GCN (AAS) or GCD (TRA) genes and in strains carrying the GCN genes on high-copy-number plasmids. These studies have led to the following conclusions. (i) HIS4 is positively regulated by the general control. (ii) At least one copy of the 5'TGACTC3' repeat at -136 is required in cis for this regulation. (iii) Both the GCN4 gene and at least one copy of the repeated sequence are required for expression at the repressed level. (iv) The open reading frames in the 5' noncoding region are not required in either cis or trans for the regulation of HIS4.
机译：已经通过使用HIS4-lacZ融合体检查了顺式和反式作用元件在HIS4表达中的作用，其中lacZ表达依赖于HIS4 5′非编码区。通过研究野生型和各种缺失及其回复子通过氨基酸生物合成的一般控制对调控的作用来定义参与调控的顺式作用序列。通过研究HIS4-lacZ融合蛋白在携带GCN（AAS）或GCD（TRA）基因突变的菌株以及携带高拷贝数质粒的GCN基因的菌株中的调控，来分析反式作用基因的作用。这些研究得出以下结论。 （i）HIS4受一般控制的积极调控。 （ii）该法规要求至少有一个在-136处的5'TGACTC3'重复序列的拷贝。 （iii）GCN4基因和重复序列的至少一个拷贝都需要以抑制水平表达。 （iv）在5'非编码区中的开放阅读框在顺式或反式中都不需要用于调节HIS4。

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