Disruption of the three cytoskeletal networks in mammalian cells does not affect transcription, translation, or protein translocation changes induced by heat shock.

W J Welch; J R Feramisco

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Disruption of the three cytoskeletal networks in mammalian cells does not affect transcription, translation, or protein translocation changes induced by heat shock.

机译：哺乳动物细胞中三个细胞骨架网络的破坏不会影响热休克引起的转录，翻译或蛋白质易位变化。

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Mammalian cells show a complex series of transcriptional and translational switching events in response to heat shock treatment which ultimately lead to the production and accumulation of a small number of proteins, the so-called heat shock (or stress) proteins. We investigated the heat shock response in both qualitative and quantitative ways in cells that were pretreated with drugs that specifically disrupt one or more of the three major cytoskeletal networks. (These drugs alone, cytochalasin E and colcemid, do not result in induction of the heat shock response.) Our results indicated that disruption of the actin microfilaments, the vimentin-containing intermediate filaments, or the microtubules in living cells does not hinder the ability of the cell to undergo an apparently normal heat shock response. Even when all three networks were simultaneously disrupted (resulting in a loose, baglike appearance of the cells), the cells still underwent a complete heat shock response as assayed by the appearance of the heat shock proteins. In addition, the major induced 72-kilodalton heat shock protein was efficiently translocated from the cytoplasm into its proper location in the nucleus and nucleolus irrespective of the condition of the three cytoskeletal elements.

机译：哺乳动物细胞响应于热激处理而显示出一系列复杂的转录和翻译转换事件，这最终导致少量蛋白质的产生和积累，即所谓的热激（或应激）蛋白质。我们以定性和定量方式研究了用药物特异性破坏三种主要细胞骨架网络中的一种或多种的药物预处理的细胞中的热休克反应。（仅这些药物，细胞松弛素E和秋水仙素，不会导致热休克反应的诱导。）我们的结果表明，肌动蛋白微丝，含波形蛋白的中间丝或活细胞中的微管的破坏并不妨碍其功能。电池单元似乎经历了正常的热冲击响应。即使同时破坏了所有三个网络（导致细胞呈松弛，袋状外观），如通过热激蛋白的出现所分析的那样，细胞仍然经历了完全的热激反应。另外，不管三种细胞骨架的情况如何，主要的诱导的72-千屈顿热休克蛋白都有效地从细胞质转移到其在核和核仁中的适当位置。

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《Molecular and Cellular Biology》 |1985年第7期|共11页
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W J Welch; J R Feramisco;
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2. IL-2 Increases Human Telomerase Reverse Transcriptase Activity Transcriptionally and Posttranslationally through Phosphatidylinositol 3'-Kinase/Akt, Heat Shock Protein 90, and Mammalian Target of Rapamycin in Transformed NK Cells. [J] . Kawauchi K, Yamada O The Journal of Immunology: Official Journal of the American Association of Immunologists . 2005,第9期

机译：IL-2通过磷脂酰肌醇3'-激酶/ Akt，热休克蛋白90和雷帕霉素的哺乳动物靶标在转化的NK细胞中转录和翻译后增加人端粒酶逆转录酶活性。
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机译：IL-2通过磷脂酰肌醇3'-激酶/ Akt，热休克蛋白90和雷帕霉素的哺乳动物靶标在转化的NK细胞中转录和翻译后增加人端粒酶逆转录酶活性。
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5. Preferential translation of Drosophila heat shock protein 70 and heat shock protein 90 mRNAs during heat shock. [D] . Ahmed, Ruhi. 2003

机译：果蝇热休克期间果蝇热休克蛋白70和热休克蛋白90 mRNA的优先翻译。
6. Disruption of the three cytoskeletal networks in mammalian cells does not affect transcription translation or protein translocation changes induced by heat shock. [O] . W J Welch, J R Feramisco 1985

机译：哺乳动物细胞中三个细胞骨架网络的破坏不会影响热休克引起的转录翻译或蛋白质易位变化。
7. Disruption of the three cytoskeletal networks in mammalian cells does not affect transcription, translation, or protein translocation changes induced by heat shock. [O] . Welch, W J, Feramisco, J R 1985

机译：哺乳动物细胞中三个细胞骨架网络的破坏不会影响热休克引起的转录，翻译或蛋白质易位变化。

Disruption of the three cytoskeletal networks in mammalian cells does not affect transcription, translation, or protein translocation changes induced by heat shock.

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