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首页> 外文期刊>Molecular and Cellular Biology >New class of polyomavirus mutant that can persist as free copies in F9 embryonal carcinoma cells.
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New class of polyomavirus mutant that can persist as free copies in F9 embryonal carcinoma cells.

机译：新型多瘤病毒突变体，可以在F9胚胎癌细胞中以自由拷贝形式持续存在。

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摘要

A small circular DNA was found extrachromosomally in a clone of F9 embryonal carcinoma (EC) cells at high copy numbers per cell. The DNA was cloned in plasmid pUC19. Restriction endonuclease analyses of the DNA indicated that the DNA (fPyF9) was a mutant of polyomavirus (Py) DNA and had a mutation in a noncoding regulatory region. There have been many reports on the isolation of Py mutants capable of replication in undifferentiated cells. However, fPyF9 was different from other Py mutants in the following aspects: it was harbored stably as a free copy at 1 X 10(4) to 5 X 10(4) copies per cell in EC cells; it replicated in undifferentiated cells better than in differentiated cells; it was extremely rearranged in the sequences of the enhancer B domain; and it carried in the enhancer B domain three copies of an exogenous sequence which does not exist in Py strain A2. From these observations, we propose a new class of Py EC mutant which has an autonomous state similar to that of plasmid and small circular DNA in host cells.
机译：在F9胚胎癌细胞（EC）细胞的克隆中，染色体外发现了一个小的环状DNA，每个细胞的拷贝数很高。将该DNA克隆到质粒pUC19中。 DNA的限制性核酸内切酶分析表明，该DNA（fPyF9）是多瘤病毒（Py）DNA的突变体，在非编码调控区具有突变。关于分离能够在未分化细胞中复制的Py突变体的报道很多。然而，fPyF9在以下方面与其他Py突变体不同：它在EC细胞中以每细胞1 X 10（4）至5 X 10（4）的拷贝形式稳定地以自由拷贝形式保存;它在未分化细胞中的复制要优于在分化细胞中的复制。它在增强子B结构域的序列中被极端重排;并且在增强子B结构域中携带了三个拷贝的外源序列，该外源序列在Py菌株A2中不存在。从这些观察结果，我们提出了一种新的Py EC突变体，其具有与宿主细胞中的质粒和小的环状DNA相似的自主状态。

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