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首页> 外文期刊>Molecular and Cellular Biology >Polylactosaminoglycan modification of a small integral membrane glycoprotein, influenza B virus NB.
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Polylactosaminoglycan modification of a small integral membrane glycoprotein, influenza B virus NB.

机译：聚乳酸氨基聚糖修饰的小整体膜糖蛋白，B型流感病毒NB。

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摘要

The structure of the carbohydrate components of NB, the small integral membrane glycoprotein of influenza B virus, was investigated. The carbohydrate chains of NB are processed from the high-mannose form (NB18) to a heterogeneous form of much higher molecular weight, designated NBp. Selection of this carbohydrate-containing form of NB with Datura stramonium lectin, its susceptibility to digestion by endo-beta-galactosidase, and determination of the size of NBp glycopeptides by gel filtration chromatography suggested that the increase in molecular weight is due to processing to polylactosaminoglycan. Investigation of the polypeptides produced by influenza B/Lee/40 virus infection of several cell types and another strain of influenza B virus suggested that the signal for modification to polylactosaminoglycan is contained in NB. Expression of mutants of NB lacking either one or both of the normal N-terminal sites of asparagine-linked glycosylation indicated that both carbohydrate chains are modified to contain polylactosaminoglycan. NBp and a small amount of unprocessed NB18 are expressed at the infected-cell surface, as determined by digestion of the surfaces of intact cells with various endoglycosidases. Unglycosylated NB, expressed either in influenza B virus-infected cells treated with tunicamycin or in cells expressing the NB mutant lacking both N-linked glycosylation sites, was expressed at the cell surface, indicating that NB does not require carbohydrate addition for transport.
机译：研究了乙型流感病毒小整体膜糖蛋白NB的碳水化合物成分的结构。 NB的碳水化合物链从高甘露糖形式（NB18）加工成分子量更高的异质形式，称为NBp。用曼陀罗粘粒凝集素选择这种含碳水化合物的NB形式，其对内β-半乳糖苷酶消化的敏感性以及通过凝胶过滤色谱法确定NBp糖肽大小的暗示表明分子量的增加是由于加工成聚乳糖胺聚糖。对几种细胞类型的B型/ Lee / 40型流感病毒感染产生的多肽和另一种B型流感病毒株的研究表明，NB中含有修饰聚乳糖胺聚糖的信号。缺少天冬酰胺连接的糖基化的正常N末端位点之一或全部的NB突变体的表达表明，两个碳水化合物链均被修饰为含有聚乳糖胺聚糖。 NBp和少量未加工的NB18在感染细胞表面表达，这是通过用各种糖苷内切酶消化完整细胞的表面确定的。未糖基化的NB在用衣霉素处理的B型流感病毒感染的细胞中或在表达缺少两个N-连接的糖基化位点的NB突变体的细胞中表达，在细胞表面表达，表明NB不需要添加碳水化合物来运输。

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