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首页> 外文期刊>Molecular and Cellular Biology >Pathway of B1-Alu expression in microinjected oocytes: Xenopus laevis proteins associated with nuclear precursor and processed cytoplasmic RNAs.
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Pathway of B1-Alu expression in microinjected oocytes: Xenopus laevis proteins associated with nuclear precursor and processed cytoplasmic RNAs.

机译：微注射卵母细胞中B1-Alu表达的途径：非洲爪蟾（Xenopus laevis）与核前体和已加工胞质RNA相关的蛋白质。

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We have previously characterized B1-Alu gene expression by microinjected Xenopus laevis oocytes. The transcription, endonucleolytic processing and its kinetics, nuclear transport kinetics, and subsequent cellular compartmentalization have been described previously (Adeniyi-Jones and Zasloff, Nature 317:81-84, 1985). Briefly, a B1-Alu gene is transcribed by RNA polymerase III to a 210-nucleotide (210nt) primary transcript which is processed to yield 135nt and 75nt RNAs. After processing, the 135nt RNA enters the cytoplasmic compartment, where it remains stable, while the 75nt RNA is degraded. In this report we characterize this pathway further and show that the RNAs involved are complexed with specific X. laevis proteins. The primary transcript was associated with an X. laevis protein of 63 kilodaltons (p63) as well as La, a protein known to be associated with RNA polymerase III transcripts. After processing, the cytoplasmic 135nt RNA remained associated only with the X. laevis p63 in the form of a small ribonucleoprotein. Human autoimmune antibodies were purified by affinity chromatography to X. laevis p63 and used to immunoprecipitate human ribonucleoprotein containing a 63-kilodalton polypeptide and small RNAs. These data suggest that Alu-analogous ribonucleoproteins and their metabolic pathways are conserved across species and provide insight as to their possible functions.
机译：我们以前已通过微注射非洲爪蟾卵母细胞表征了B1-Alu基因表达。转录，核酸内切加工及其动力学，核转运动力学和随后的细胞区室化以前已有描述（Adeniyi-Jones and Zasloff，Nature 317：81-84，1985）。简而言之，B1-Alu基因被RNA聚合酶III转录为一个210核苷酸（210nt）的初级转录本，该转录本被加工成135nt和75nt的RNA。加工后，135nt RNA进入细胞质区室，在那里保持稳定，而75nt RNA被降解。在本报告中，我们进一步表征了该途径，并表明所涉及的RNA与特定的X. laevis蛋白复合。初级转录本与63千道尔顿的X. laevis蛋白（p63）以及La（已知与RNA聚合酶III转录本相关的蛋白）有关。加工后，胞质135nt RNA仅以小核糖核蛋白形式与X. laevis p63结合。通过对X. laevis p63的亲和层析纯化人自身免疫抗体，并用于免疫沉淀包含63千达尔顿多肽和小RNA的人核糖核蛋白。这些数据表明，Alu类似的核糖核蛋白及其代谢途径在物种间是保守的，并提供了有关其可能功能的见解。

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