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首页> 外文期刊>Molecular and Cellular Biology >The human L-myc gene encodes multiple nuclear phosphoproteins from alternatively processed mRNAs.
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The human L-myc gene encodes multiple nuclear phosphoproteins from alternatively processed mRNAs.

机译：人类L-myc基因编码来自交替加工的mRNA的多种核磷蛋白。

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摘要

The human proto-oncogene L-myc generates at least four different mRNAs by alternative RNA processing. We have identified two phosphorylated L-myc proteins with molecular masses of 60,000 and 66,000 daltons [p60L-myc(human) and p66L-myc(human)] in a small-cell carcinoma line expressing high levels of L-myc mRNA. These proteins have a short half-life and are localized to the nuclear matrix fraction, as previously reported for the c-myc and N-myc proteins. In vitro translation experiments demonstrated that both the p60 and p66 species are encoded by a 3.9-kilobase (kb) mRNA which retains intron 1, while only the p60 protein is translated from a 3.6-kb L-myc mRNA which has had intron 1 removed. While L-myc proteins [p32L-myc(human) and p37L-myc(human)] could be synthesized in vitro from 2.2-kb mRNA templates, no such proteins were detected by immunoprecipitation in vivo. These observations suggest that alternative RNA processing of the L-myc transcript could play a role in determining the steady-state levels of the p60L-myc and p66L-myc proteins.
机译：人类原癌基因L-myc通过替代RNA加工产生至少四个不同的mRNA。我们已经鉴定了两种磷酸化的L-myc蛋白，其分子量分别为60,000和66,000道尔顿[p60L-myc（人类）和p66L-myc（人类）]，它们表达高水平的L-myc mRNA。这些蛋白质的半衰期短，并且位于核基质部分，如先前报道的c-myc和N-myc蛋白质。体外翻译实验表明，p60和p66物种均由保留内含子1的3.9碱基（kb）mRNA编码，而从已去除内含子1的3.6 kb L-myc mRNA中仅翻译了p60蛋白。 。虽然L-myc蛋白[p32L-myc（人类）和p37L-myc（人类）]可以在体外从2.2-kb mRNA模板合成，但在体内通过免疫沉淀未检测到此类蛋白质。这些观察结果表明，L-myc转录本的替代RNA处理可能在确定p60L-myc和p66L-myc蛋白的稳态水平中起作用。

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