• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >The muscle creatine kinase gene is regulated by multiple upstream elements, including a muscle-specific enhancer.
【24h】

The muscle creatine kinase gene is regulated by multiple upstream elements, including a muscle-specific enhancer.

机译：肌酸肌酸激酶基因受多个上游元件的调节，包括肌肉特异性增强子。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Muscle creatine kinase (MCK) is induced to high levels during skeletal muscle differentiation. We have examined the upstream regulatory elements of the mouse MCK gene which specify its activation during myogenesis in culture. Fusion genes containing up to 3,300 nucleotides (nt) of MCK 5' flanking DNA in various positions and orientations relative to the bacterial chloramphenicol acetyltransferase (CAT) structural gene were transfected into cultured cells. Transient expression of CAT was compared between proliferating and differentiated MM14 mouse myoblasts and with nonmyogenic mouse L cells. The major effector of high-level expression was found to have the properties of a transcriptional enhancer. This element, located between 1,050 and 1,256 nt upstream of the transcription start site, was also found to have a major influence on the tissue and differentiation specificity of MCK expression; it activated either the MCK promoter or heterologous promoters only in differentiated muscle cells. Comparisons of viral and cellular enhancer sequences with the MCK enhancer revealed some similarities to essential regions of the simian virus 40 enhancer as well as to a region of the immunoglobulin heavy-chain enhancer, which has been implicated in tissue-specific protein binding. Even in the absence of the enhancer, low-level expression from a 776-nt MCK promoter retained differentiation specificity. In addition to positive regulatory elements, our data provide some evidence for negative regulatory elements with activity in myoblasts. These may contribute to the cell type and differentiation specificity of MCK expression.
机译：在骨骼肌分化过程中，肌酸肌酸激酶（MCK）被诱导至高水平。我们已经检查了小鼠MCK基因的上游调节元件，其指定了其在培养的肌发生过程中的激活。将相对于细菌氯霉素乙酰基转移酶（CAT）结构基因处于各种位置和方向的，包含多达3,300个核苷酸的MCK 5'侧翼DNA的融合基因转染到培养细胞中。在增殖和分化的MM14小鼠成肌细胞与非成肌小鼠L细胞之间比较了CAT的瞬时表达。发现高水平表达的主要效应子具有转录增强子的特性。还发现该元件位于转录起始位点上游1,050至1,256 nt之间，对MCK表达的组织和分化特异性有重大影响。它仅在分化的肌肉细胞中激活MCK启动子或异源启动子。病毒和细胞增强子序列与MCK增强子的比较揭示了与猿猴病毒40增强子的基本区域以及免疫球蛋白重链增强子的区域有一些相似性，这与组织特异性蛋白结合有关。即使没有增强子，来自776-nt MCK启动子的低水平表达仍保留了分化特异性。除了积极的调控元素外，我们的数据还为成肌细胞具有活性的负调控元素提供了一些证据。这些可能有助于MCK表达的细胞类型和分化特异性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号