Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

V K Parnaik; P K Kennady

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Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

机译：从SP6质粒生成的mRNA体外翻译的蛋白质的核转运。

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A sensitive and versatile assay is described for the nuclear transport of 35S-labeled proteins obtained by the in vitro translation of SP6 plasmid-generated mRNAs. A specific nuclear accumulation of greater than 20-fold is observed for the transformation-related nuclear proteins, p53 and E1b, and the nuclear enzyme, thymidine kinase, whereas transport of the nonnuclear proteins, dihydrofolate reductase and simian virus 40 small t antigen, is negligible within 30 min.

机译：描述了一种灵敏且用途广泛的测定方法，用于通过SP6质粒生成的mRNA的体外翻译获得的35S标记蛋白的核转运。观察到与转化相关的核蛋白p53和E1b以及核酶胸苷激酶的特异性核蓄积大于20倍，而无核蛋白，二氢叶酸还原酶和猿猴病毒40小t抗原的转运是20倍。在30分钟内可忽略不计。

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《Molecular and Cellular Biology》 |1990年第3期|共6页
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V K Parnaik; P K Kennady;
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中图分类细胞生物学;
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1. In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins. [J] . D D Newmeyer, D R Finlay, D J Forbes Journal of cell biology . 1986,第6期

机译：荧光核蛋白的体外转运和非核蛋白的排除。
2. In vitro characterization of rice importin β1: molecular interaction with nuclear transport factors and mediation of nuclear protein import [J] . Imamoto Naoko, Jiang Chang-Jie, Matsuki Rikyu, FEBS Letters . 1998,第1a2期

机译：水稻importinβ1的体外表征：与核转运因子的分子相互作用和核蛋白进口的介导
3. In vitro characterization of rice importin β1: molecular interaction with nuclear transport factors and mediation of nuclear protein import [J] . Imamoto Naoko, Jiang Chang-Jie, Matsuki Rikyu, FEBS Letters . 1998,第1a2期

机译：水稻importinβ1的体外表征：与核转运因子的分子相互作用和核蛋白进口的介导
4. A systems biology approach to understanding in-vitro protein translation reveals a biosignature of inhibitory cold-shock proteins [C] . Sushmita Mimi Roy, Sunil Bajad, Evan Green, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：理解体外蛋白翻译的系统生物学方法揭示了抑制性冷冲击蛋白的生物炎
5. The eIF2alpha kinases GCN2 and PERK regulate phosphorylation of TOR target proteins and differential translation of mRNAs. [D] . Dang Do, An Ngoc. 2008

机译：eIF2alpha激酶GCN2和PERK调节TOR靶蛋白的磷酸化和mRNA的差异翻译。
6. Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs. [O] . V K Parnaik, P K Kennady 1990

机译：从SP6质粒生成的mRNA体外翻译的蛋白质的核转运。
7. Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs. [O] . Parnaik, V K, Kennady, P K 1990

机译：从SP6质粒生成的mRNA体外翻译的蛋白质的核转运。

Nuclear transport of proteins translated in vitro from SP6 plasmid-generated mRNAs.

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