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首页> 外文期刊>Molecular and Cellular Biology >Expression of a gene family in the dimorphic fungus Mucor racemosus which exhibits striking similarity to human ras genes.
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Expression of a gene family in the dimorphic fungus Mucor racemosus which exhibits striking similarity to human ras genes.

机译：基因家族在双态真菌Mucor racemosus中的表达与人类ras基因具有惊人的相似性。

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Sporulation, spore germination, and yeast-hypha dimorphism in the filamentous fungus Mucor racemosus provide useful model systems to study cell development in eucaryotic cells. Three RAS genes (MRAS1, MRAS2, and MRAS3) from M. racemosus have been cloned, and their nucleotide sequences have been determined. The predicted amino acid sequences and the sizes of the three MRAS proteins exhibit a high degree of similarity with other ras proteins, including that encoded by H-ras, which have been implicated in regulation of proliferation and development in eucaryotic cells by mediating signal transduction pathways. The MRAS proteins show conservation of functional domains proposed for ras proteins, including guanine nucleotide interaction domains, an effector domain, a binding epitope for neutralizing antibody Y13-259, and the COOH-terminal CAAX box, which is a site of thiocylation and membrane attachment. Amino acid sequences unique to each MRAS protein occur adjacent to the CAAX box, consistent with the location of the hypervariable region in other ras proteins. Northern (RNA) analysis was used to study expression of the three MRAS genes in relation to cell development. Gene-specific probes for two of these genes, MRAS1 and MRAS3, hybridized to different 1.3-kb mRNA transcripts. The accumulation of these transcripts depended on the developmental stage, and this pattern was different between the two MRAS genes. No transcript for MRAS2 was detected in the developmental stages examined. The unique patterns of MRAS transcript accumulation suggest that individual MRAS genes and proteins may play distinct roles in cell growth or development.
机译：丝状真菌Mucor racemosus中的孢子形成，孢子萌发和酵母菌丝多态性为研究真核细胞的发育提供了有用的模型系统。已克隆了来自总状分枝杆菌的三个RAS基因（MRAS1，MRAS2和MRAS3），并确定了它们的核苷酸序列。三个MRAS蛋白的预测氨基酸序列和大小与其他ras蛋白（包括由H-ras编码的蛋白）具有高度相似性，这些蛋白通过介导信号转导通路参与调节真核细胞的增殖和发育。 MRAS蛋白显示为ras蛋白提议的功能结构域的保守性，包括鸟嘌呤核苷酸相互作用结构域，效应子结构域，中和抗体Y13-259的结合表位和COOH末端CAAX框，该框是巯基化和膜附着的位点。每个MRAS蛋白特有的氨基酸序列出现在CAAX框附近，与其他ras蛋白中高变区的位置一致。 Northern（RNA）分析用于研究与细胞发育有关的三个MRAS基因的表达。这些基因中的两个MRAS1和MRAS3的基因特异性探针与不同的1.3-kb mRNA转录本杂交。这些转录本的积累取决于发育阶段，并且两个MRAS基因之间的这种模式是不同的。在检查的发育阶段未检测到MRAS2的转录本。 MRAS转录物积累的独特模式表明，单个MRAS基因和蛋白质可能在细胞生长或发育中发挥不同的作用。

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