• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Transient expression of a winged-helix protein, MNF-beta, during myogenesis.
【24h】

Transient expression of a winged-helix protein, MNF-beta, during myogenesis.

机译：在成肌过程中有翼螺旋蛋白MNF-β的瞬时表达。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel winged-helix transcription factor, MNF-beta, is expressed coincidentally with cell cycle withdrawal and differentiation of skeletal myogenic cells. MNF-beta is closely related to the myocyte nuclear factor (MNF) protein previously described (now termed MNF-alpha), but expression of the two isoforms is differentially regulated, and they exhibit distinctive functional properties with respect to DNA binding in vitro and transcriptional regulatory activity in transient-transfection assays. A DNA sequence motif binding MNF-beta with high affinity was selected from a library of random oligonucleotides and was found to be similar to but distinct from the cognate binding site for HNF-3beta, a more distantly related winged-helix protein. The temporal pattern of MNF-beta expression and the presence of MNF binding motifs within conserved promoter elements of several genes that modulate cell cycle progression support a working hypothesis that MNF proteins may modulate proliferation of myogenic precursor cells during development and muscle regeneration.
机译：一种新型的有翼螺旋转录因子，MNF-β，与骨骼肌成肌细胞的细胞周期停滞和分化同时表达。 MNF-beta与先前描述的肌细胞核因子（MNF）蛋白（现称为MNF-alpha）密切相关，但是两种同工型的表达受到差异调节，并且它们在体外和转录DNA结合方面表现出独特的功能特性瞬时转染测定中的调节活性。从随机寡核苷酸文库中选择了具有高亲和力的结合MNF-β的DNA序列基序，发现该序列与HNF-3β（一种更远距离相关的有翼螺旋蛋白）的同源结合位点相似但又不同。 MNF-β表达的时间模式和调节细胞周期进程的几个基因的保守启动子中MNF结合基序的存在支持了一个有效的假设，即MNF蛋白可能在发育和肌肉再生过程中调节成肌前体细胞的增殖。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号