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首页> 外文期刊>Molecular and Cellular Biology >The casein kinase II beta subunit binds to Mos and inhibits Mos activity.
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The casein kinase II beta subunit binds to Mos and inhibits Mos activity.

机译：酪蛋白激酶IIβ亚基与Mos结合并抑制Mos活性。

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Mos is a germ cell-specific serine/threonine kinase and is required for Xenopus oocyte maturation. Active Mos stimulates a mitogen-activated protein kinase (MAPK) by directly phosphorylating and activating MAPK kinase (MKK). We report here that the Xenopus homolog of the beta subunit of casein kinase II (CKII beta) binds to and regulates Mos. The Mos-interacting region of CKII beta was mapped to the C terminus. Mos bound to CKII beta in somatic cells ectopically expressing Mos and CKII beta as well as in unfertilized Xenopus eggs. CKII beta inhibited Mos-mediated MAPK activation in rabbit reticulocyte lysates and repressed MKK activation by v-Mos in a coupled kinase assay. In addition, microinjection of CKII beta mRNA into Xenopus oocytes inhibited progesterone-induced meiotic maturation and MAPK activation, presumably by binding of CKII beta to Mos and thereby inhibiting MAPK activation. Moreover, this inhibitory phenotype could be rescued by another protein that binds to CKII beta, CKII alpha. The ability of ectopic CKII beta to inhibit meiotic maturation and the detection of a complex between endogenous Mos and CKII beta suggest that CKII beta may act as an inhibitor of Mos during oocyte maturation, perhaps setting a threshold beyond which Mos protein must accumulate before it can activate the MAPK pathway.
机译：Mos是生殖细胞特有的丝氨酸/苏氨酸激酶，是非洲爪蟾卵母细胞成熟所必需的。 Active Mos通过直接磷酸化和激活MAPK激酶（MKK）来刺激有丝分裂原激活的蛋白激酶（MAPK）。我们在这里报告酪蛋白激酶II（CKII beta）的β亚基的爪蟾同系物绑定并调节莫斯。 CKII beta的Mos相互作用区域被映射到C末端。在异位表达Mos和CKII beta的体细胞以及未受精的非洲爪蟾卵中，Mos与CKII beta结合。在偶联激酶测定中，CKII beta抑制了兔网织红细胞裂解物中Mos介导的MAPK活化，并通过v-Mos抑制了MKK活化。另外，向爪蟾卵母细胞显微注射CKII beta mRNA可抑制孕激素诱导的减数分裂成熟和MAPK活化，大概是通过CKII beta与Mos的结合从而抑制了MAPK活化。此外，这种抑制表型可以通过与CKII beta结合的另一种蛋白CKII alpha挽救。异位CKII beta抑制减数分裂成熟的能力以及内源性Mos和CKII beta之间复合物的检测表明CKII beta可能在卵母细胞成熟过程中起Mos抑制剂的作用，也许设定了一个阈值，超过此阈值Mos蛋白必须能够积累激活MAPK途径。

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