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首页> 外文期刊>Molecular and Cellular Biology >Ultrabithorax and Antennapedia 5' untranslated regions promote developmentally regulated internal translation initiation.
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Ultrabithorax and Antennapedia 5' untranslated regions promote developmentally regulated internal translation initiation.

机译：Ultrabithorax和Antennapedia 5'非翻译区促进发育受调控的内部翻译起始。

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The 5' untranslated regions (UTRs) of the Drosophila Ubx and Antp genes were tested for their ability to promote cap-independent translation initiation. The Ubx and the Antp 5' UTR were inserted between the CAT and lacZ coding sequences in a dicistronic gene and tested for IRES activity in transgenic Drosophila. Northern analysis of the mRNAs showed the presence of the predicted full-length dicistronic mRNAs. High CAT activity was expressed from the first cistron from all of the dicistronic constructs introduced into the fly genome. The dicistronic transgenic strains bearing the Ubx and Antp IRES elements expressed significant levels of beta-galactosidase (betaGAL) from the second cistron whereas little or no betaGAL was expressed in the controls lacking the IRESs. In situ analysis of betaGAL expression in the transgenic strains indicates that expression of the second cistron is spatially and temporally regulated. Although the developmental patterns of expression directed by the Antp and Ubx IRESs overlap, they exhibit several differences indicating that these IRESs are not functionally equivalent.
机译：测试果蝇Ubx和Antp基因的5'非翻译区（UTRs）促进不依赖于帽的翻译起始的能力。将Ubx和Antp 5'UTR插入双顺反子基因中的CAT和lacZ编码序列之间，并测试了转基因果蝇中的IRES活性。 RNA的Northern分析表明存在预测的全长双顺反子mRNA。从引入飞蝇基因组的所有双顺反子构建体的第一个顺反子表达了高CAT活性。带有Ubx和Antp IRES元件的双顺反子转基因菌株从第二个顺反子表达了显着水平的β-半乳糖苷酶（betaGAL），而在缺乏IRES的对照中几乎没有表达betaGAL。转基因菌株中βGAL表达的原位分析表明第二个顺反子的表达在空间和时间上都受到调控。尽管由Antp和Ubx IRES指导的表达的发育模式重叠，但它们表现出一些差异，表明这些IRES在功能上不相同。

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