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首页> 外文期刊>Molecular and Cellular Biology >Regulation of the cfos serum response element by C/EBPbeta.
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Regulation of the cfos serum response element by C/EBPbeta.

机译：C / EBPbeta对cfos血清反应元件的调节。

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Serum response element binding protein (SRE BP) is a novel binding factor present in nuclear extracts of avian and NIH 3T3 fibroblasts which specifically binds to the cfos SRE within a region overlapping and immediately 3' to the CArG box. Site-directed mutagenesis combined with transfection experiments in NIH 3T3 cells showed that binding of both serum response factor (SRF) and SRE BP is necessary for maximal serum induction of the SRE. In this study, we have combined size fractionation of the SRE BP DNA binding activity with C/EBPbeta antibodies to demonstrate that homodimers and heterodimers of p35C/EBPbeta (a transactivator) and p20C/EBPbeta (a repressor) contribute to the SRE BP complex in NIH 3T3 cells. Transactivation of the SRE by p35C/EBPbeta is dependent on SRF binding but not ternary complex factor (TCF) formation. Both p35C/EBPbeta and p20C/EBPbeta bind to SRF in vitro via a carboxy-terminal domain that probably does not include the leucine zipper. Moreover, SRE mutants which retain responsiveness to the TCF-independent signaling pathway bind SRE BP in vitro with affinities that are nearly identical to that of the wild-type SRE, whereas mutant SRE.M, which is not responsive to the TCF-independent pathway, has a nearly 10-fold lower affinity for SRE BP. We propose that C/EBPbeta may play a role in conjunction with SRF in the TCF-independent signaling pathway for SRE activation.
机译：血清反应元件结合蛋白（SRE BP）是存在于禽类和NIH 3T3成纤维细胞核提取物中的新型结合因子，可在重叠区域内并紧靠CArG盒的3'内特异性结合cfos SRE。定点诱变结合NIH 3T3细胞中的转染实验表明，血清反应因子（SRF）和SRE BP的结合对于最大程度地诱导SRE产生血清是必需的。在这项研究中，我们将SRE BP DNA结合活性的大小分级与C / EBPbeta抗体相结合，以证明p35C / EBPbeta（反式激活物）和p20C / EBPbeta（阻遏物）的同二聚体和异二聚体有助于SRE BP复合体的形成。 NIH 3T3细胞。 p35C / EBPbeta对SRE的反式激活取决于SRF结合，而不取决于三元复合因子（TCF）的形成。 p35C / EBPbeta和p20C / EBPbeta在体外均通过可能不包含亮氨酸拉链的羧基末端结构域与SRF结合。此外，保留对TCF非依赖性信号途径有反应性的SRE突变体在体外以与野生型SRE亲和力几乎相同的亲和力与SRE BP结合，而对TCF非依赖性途径无响应的SRE.M突变体，对SRE BP的亲和力低近10倍。我们建议，C / EBPbeta可能在SRE激活的TCF独立信号通路中与SRF结合发挥作用。

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