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首页> 外文期刊>Molecular and Cellular Biology >Phosphorylation-Independent Inhibition of Cdc28p by the Tyrosine Kinase Swe1p in the Morphogenesis Checkpoint
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Phosphorylation-Independent Inhibition of Cdc28p by the Tyrosine Kinase Swe1p in the Morphogenesis Checkpoint

机译：酪氨酸激酶Swe1p在形态发生检查点的磷酸化非依赖性抑制Cdc28p。

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The morphogenesis checkpoint in budding yeast delays cell cycle progression in G2 when the actin cytoskeleton is perturbed, providing time for cells to complete bud formation prior to mitosis. Checkpoint-induced G2 arrest involves the inhibition of the master cell cycle regulatory cyclin-dependent kinase, Cdc28p, by the Wee1 family kinase Swe1p. Results of experiments using a nonphosphorylatable CDC28Y19F allele suggested that the checkpoint stimulated two inhibitory pathways, one that promoted phosphorylation at tyrosine 19 (Y19) and a poorly characterized second pathway that did not require Cdc28p Y19 phosphorylation. We present the results from a genetic screen for checkpoint-defective mutants that led to the repeated isolation of the dominant CDC28E12K allele that is resistant to Swe1p-mediated inhibition. Comparison of this allele with the nonphosphorylatable CDC28Y19F allele suggested that Swe1p is still able to inhibit CDC28Y19F in a phosphorylation-independent manner and that both the Y19 phosphorylation-dependent and -independent checkpoint pathways in fact reflect Swe1p inhibition of Cdc28p. Remarkably, we found that a Swe1p mutant lacking catalytic activity could significantly delay the cell cycle in vivo during a physiological checkpoint response, even when expressed at single copy. The finding that a Wee1 family kinase expressed at physiological levels can inhibit a nonphosphorylatable cyclin-dependent kinase has broad implications for many checkpoint studies using such mutants in other organisms.
机译：当肌动蛋白细胞骨架受到扰动时，发芽酵母中的形态发生检查点会延迟G 2 的细胞周期进程，为细胞在有丝分裂之前完成芽的形成提供了时间。检查点诱导的G 2 阻滞涉及Wee1家族激酶Swe1p对主细胞周期调节细胞周期蛋白依赖性激酶Cdc28p的抑制作用。使用不可磷酸化的 CDC28 Y19F 等位基因的实验结果表明，该检查点刺激了两个抑制途径，一个在酪氨酸19（Y19）处促进了磷酸化，而一个表征较差的第二个途径却在不需要Cdc28p Y19磷酸化。我们提出了检查点缺陷型突变体的遗传筛选结果，该突变体导致了对Swe1p介导的抑制具有抗性的显性 CDC28 E12K 等位基因的反复分离。将该等位基因与不可磷酸化的 CDC28 Y19F 等位基因进行比较表明，Swe1p仍然能够抑制 CDC28 Y19F 。磷酸化无关的方式，以及Y19磷酸化依赖性和非依赖性检查点途径实际上都反映了Swe1p对Cdc28p的抑制。值得注意的是，我们发现，缺乏催化活性的Swe1p突变体即使在单拷贝表达时，也可以在生理学检查点反应期间显着延迟体内细胞周期。在生理水平表达的Wee1家族激酶可以抑制不可磷酸化的细胞周期蛋白依赖性激酶的发现，对于在其他生物中使用此类突变体的许多检查点研究具有广泛的意义。

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