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>Down-Regulation of RpS21, a Putative Translation Initiation Factor Interacting with P40, Produces Viable Minute Imagos and Larval Lethality with Overgrown Hematopoietic Organs and Imaginal Discs
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Down-Regulation of RpS21, a Putative Translation Initiation Factor Interacting with P40, Produces Viable Minute Imagos and Larval Lethality with Overgrown Hematopoietic Organs and Imaginal Discs
Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minutephenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista(sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth inrpS21-deficient larvae, whereas viable combinations betweenrpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs.
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机译：果蝇核糖体蛋白S21基因（ rpS21 ）的下调导致显性 Minute 表型显性弱，并隐性地产生造血器官的大量增生，幼虫发育过程中假牙盘适度过度生长。在这里，我们显示S21蛋白（RpS21）结合到盐不稳定的天然40S核糖体亚基中，并且不在多核糖体中，这表明它充当翻译起始因子而不是核心核糖体蛋白。 RpS21可与P40强烈相互作用，P40是由 stubarista （ sta ）基因编码的核糖体外围蛋白。遗传学研究表明，P40的过表达大大增强了 rpS21 缺陷幼虫的假想椎间盘过度生长，而 rpS21 和 sta 之间的可行组合会影响刷毛的形态。 ，触角和针叶科。这些数据证明了翻译机制各组成部分之间的强相互作用，并表明它们的低表达削弱了造血器官和假想盘中细胞增殖的控制。
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