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Absence of Apparent Phenotype in Mice Lacking Cdc25C Protein Phosphatase

机译：缺乏Cdc25C蛋白磷酸酶的小鼠中没有明显的表型。

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The Cdc25 family of protein phosphatases positively regulate the cell division cycle by activating cyclin-dependent protein kinases. In humans and rodents, three Cdc25 family members denoted Cdc25A, -B, and -C have been identified. The murine forms of Cdc25 exhibit distinct patterns of expression both during development and in adult mouse tissues. In order to determine unique contributions made by the Cdc25C protein phosphatase to embryonic and adult cell cycles, mice lacking Cdc25C were generated. We report thatCdc25C ?/? mice are viable and do not display any obvious abnormalities. Among adult tissues in whichCdc25C is detected, its transcripts are most abundant in testis, followed by thymus, ovary, spleen, and intestine. Mice lackingCdc25C were fertile, indicating that Cdc25Cdoes not contribute an essential function during spermatogenesis or oogenesis in the mouse. T- and B-cell development was also found to be normal in Cdc25C ?/? mice, andCdc25C ?/? mouse splenic T and B cells exhibited normal proliferative responses in vitro. Finally, the phosphorylation status of Cdc2, the timing of entry into mitosis, and the cellular response to DNA damage were unperturbed in mouse embryo fibroblasts lacking Cdc25C. These findings indicate thatCdc25A and/or Cdc25B may compensate for loss ofCdc25C in the mouse.
机译：Cdc25家族的蛋白磷酸酶通过激活细胞周期蛋白依赖性蛋白激酶来积极调节细胞分裂周期。在人类和啮齿动物中，已识别出三个Cdc25家族成员，分别表示为Cdc25A，-B和-C。 Cdc25的鼠类形式在发育期间和成年小鼠组织中均表现出不同的表达模式。为了确定Cdc25C蛋白磷酸酶对胚胎和成年细胞周期的独特贡献，生成了缺乏 Cdc25C 的小鼠。我们报告 Cdc25C ？/？小鼠是可行的，并且没有显示任何明显的异常。在检测到 Cdc25C 的成人组织中，其转录本在睾丸中含量最高，其次是胸腺，卵巢，脾脏和肠道。缺乏 Cdc25C 的小鼠可育，表明 Cdc25C 在小鼠精子发生或卵子生成过程中不发挥重要作用。还发现 Cdc25C ？/？小鼠和 Cdc25C ？/？<小鼠脾脏T和B细胞在体外表现出正常的增殖反应。最后，在缺乏 Cdc25C 的小鼠胚胎成纤维细胞中，Cdc2的磷酸化状态，进入有丝分裂的时间以及对DNA损伤的细胞反应均不受干扰。这些发现表明 Cdc25A 和/或 Cdc25B 可能弥补了小鼠 Cdc25C 的损失。

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