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首页> 外文期刊>Molecular and Cellular Biology >A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.
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A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis.

机译：裂变酵母中的泛素结合酶，对有丝分裂后期的发生至关重要。

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A cDNA encoding a ubiquitin-conjugating enzyme designated UbcP4 in fission yeast was isolated. Disruption of its genomic gene revealed that it was essential for cell viability. In vivo depletion of the UbcP4 protein demonstrated that it was necessary for cell cycle progression at two phases, G2/M and metaphase/anaphase transitions. The G2 arrest of UbcP4-depleted cells was dependent upon chk1, which mediates checkpoint pathway. UbcP4-depleted cells arrested at metaphase had condensed chromosomes but were defective in separation. However, septum formation and cytokinesis were not restrained during the metaphase arrest. Overexpression of UbcP4 specifically rescued the growth defect of cut9ts cells at a restrictive temperature. cut9 encodes a component of the anaphase-promoting complex (APC) which is required for chromosome segregation at anaphase and moreover is defined as cyclin-specific ubiquitin ligase. Cdc13, a mitotic cyclin in fission yeast, was accumulated in the UbcP4-depleted cells. These results strongly suggested that UbcP4 is a ubiquitin-conjugating enzyme working in conjunction with APC and mediates the ubiquitin pathway for degradation of "sister chromatid holding protein(s)" at the onset of anaphase and possibly of mitotic cyclin at the exit of mitosis.
机译：分离了在裂变酵母中编码称为UbcP4的泛素结合酶的cDNA。其基因组基因的破坏表明，它对细胞活力至关重要。 UbcP4蛋白的体内耗竭表明，细胞周期在两个阶段（G2 / M和中期/后期转换）进行是必要的。 UbcP4耗尽的细胞的G2逮捕取决于chk1，其介导检查点途径。在中期停滞的UbcP4耗尽的细胞具有浓缩的染色体，但在分离方面有缺陷。然而，在中期停搏期间，隔膜的形成和胞质分裂不受限制。在限制温度下，UbcP4的过度表达可以挽救cut9ts细胞的生长缺陷。 cut9编码后期促进染色体分离所必需的后期促进复合物（APC）的一个组成部分，而且被定义为细胞周期蛋白特异性泛素连接酶。 Cdc13，裂变酵母中的有丝分裂细胞周期蛋白，积累在贫化UbcP4的细胞中。这些结果强烈表明，UbcP4是一种泛素结合酶，可与APC协同工作，并在后期开始时介导泛素途径降解“姐妹染色单体固持蛋白”，并可能在有丝分裂开始时降解有丝分裂细胞周期蛋白。

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