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首页> 外文期刊>Molecular and Cellular Biology >Retroviral insertions 90 kilobases proximal to the Evi-1 myeloid transforming gene activate transcription from the normal promoter.
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Retroviral insertions 90 kilobases proximal to the Evi-1 myeloid transforming gene activate transcription from the normal promoter.

机译：Evi-1髓样转化基因近端90 kb处的逆转录病毒插入激活了正常启动子的转录。

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The inappropriate production of the Evi-1 zinc finger protein occurs in retrovirus-induced murine myeloid leukemias and human acute myelogenous leukemias. In murine leukemias, expression of the Evi-1 gene is associated with retroviral insertions either in the Evi-1 locus, which is immediately 5' of the coding region of the gene, or in the genetically linked Cb-1/fim-3 locus. In these studies, we demonstrate by chromosomal walking and pulse field electrophoresis that the Cb-1/fim-3 locus is located 90 kb 5' of the Evi-1 locus. Primary structure analysis of Evi-1 cDNA clones from a Cb-1/fim-3 rearranged cell line (DA-3) demonstrates that transcription initiates 5' of the Evi-1 locus and that the first noncoding exon of the gene is 681 bp larger than previously defined. S1 nuclease protection studies reveal multiple transcription initiation sites within this region. Comparable transcriptional initiation sites were identified in RNA from kidney and ovary, in which the gene is normally expressed, suggesting that retroviral insertions in the Cb-1/fim-3 locus activate transcription from the normal promoter. In one myeloid cell line (DA-3), a single long terminal repeat (LTR) is present in the Cb-1/fim-3 locus. No stable transcripts were detectable from this LTR. In cells with retroviral insertions in the Cb-1/fim-3 locus, one allele of the Evi-1 locus becomes hypermethylated in the 5' region of the gene. Together, these results are most consistent with an LTR-mediated, long-range cis activation of Evi-1 gene expression.
机译：Evi-1锌指蛋白的不适当产生发生在逆转录病毒诱导的鼠骨髓性白血病和人急性髓性白血病中。在鼠白血病中，Evi-1基因的表达与逆转录病毒插入相关，或者与Evi-1基因座（紧接该基因编码区的5'）或遗传连接的Cb-1 / fim-3基因座有关。在这些研究中，我们通过染色体行走和脉冲场电泳证明Cb-1 / fim-3基因座位于Evi-1基因座的90 kb 5'处。来自Cb-1 / fim-3重排细胞系（DA-3）的Evi-1 cDNA克隆的一级结构分析表明，转录启动了Evi-1基因座的5'端，该基因的第一个非编码外显子为681 bp比以前定义的大。 S1核酸酶保护研究揭示了该区域内的多个转录起始位点。在正常表达该基因的肾脏和卵巢中，在RNA中鉴定出了可比的转录起始位点，表明在Cb-1 / fim-3基因座中的逆转录病毒插入激活了正常启动子的转录。在一个髓样细胞系（DA-3）中，Cb-1 / fim-3基因座中存在一个长末端重复序列（LTR）。从该LTR中未检测到稳定的转录本。在Cb-1 / fim-3基因座中具有逆转录病毒插入的细胞中，Evi-1基因座的一个等位基因在基因的5'区域变得高度甲基化。总之，这些结果与LTR介导的Evi-1基因表达的远程顺式激活最一致。

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