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首页> 外文期刊>Molecular and Cellular Biology >SRN1, a yeast gene involved in RNA processing, is identical to HEX2/REG1, a negative regulator in glucose repression.
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SRN1, a yeast gene involved in RNA processing, is identical to HEX2/REG1, a negative regulator in glucose repression.

机译：SRN1是参与RNA加工的酵母基因，与HEX2 / REG1（葡萄糖抑制的负调节剂）相同。

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摘要

The yeast RNA1 gene encodes a cytosolic protein that affects pre-tRNA splicing, pre-rRNA processing, the production of mRNA, and the export of RNA from the nucleus to the cytosol. In an attempt to understand how the RNA1 protein affects such a diverse set of processes, we sought second-site suppressors of a mutation, rna1-1, of the RNA1 locus. Mutations in a single complementation group were obtained. These lesions proved to be in the same gene, SRN1, identified previously in a search for second-site suppressors of mutations that affect the removal of intervening sequences from pre-mRNAs. The SRN1 gene was mapped, cloned, and sequenced. DNA sequence analysis and the phenotype of disruption mutations showed that, surprisingly, SRN1 is identical to HEX2/REG1, a gene that negatively regulates glucose-repressible genes. Interestingly, SRN1 is not a negative regulator of RNA1 at the transcriptional, translational, or protein stability level. However, SRN1 does regulate the level of two newly discovered antigens, p43 and p70, one of which is not glucose repressible. These studies for the first time link RNA processing and carbon catabolite repression.
机译：酵母RNA1基因编码一种胞质蛋白，可影响tRNA之前的剪接，rRNA之前的加工，mRNA的产生以及RNA从细胞核到细胞质的输出。为了试图了解RNA1蛋白如何影响如此多样的过程，我们寻求了RNA1基因座rna1-1突变的第二位抑制剂。获得单个互补组中的突变。这些损伤被证明是在相同的基因SRN1中，该基因先前是在寻找影响突变位点的第二位抑制剂时发现的，这些突变位点影响从前mRNA去除插入序列。 SRN1基因被定位，克隆和测序。 DNA序列分析和破坏突变的表型表明，令人惊讶的是，SRN1与HEX2 / REG1相同，后者是一种负调节葡萄糖可抑制基因的基因。有趣的是，SRN1在转录，翻译或蛋白质稳定性水平上都不是RNA1的负调节剂。但是，SRN1确实调节了两种新发现的抗原p43和p70的水平，其中一种不是葡萄糖可抑制的。这些研究首次将RNA加工与碳分解代谢物阻遏联系在一起。

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