Mutations in a 19-amino-acid hydrophobic region of the yeast cytochrome c1 presequence prevent sorting to the mitochondrial intermembrane space.

R E Jensen; S Schmidt; R J Mark

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Mutations in a 19-amino-acid hydrophobic region of the yeast cytochrome c1 presequence prevent sorting to the mitochondrial intermembrane space.

机译：酵母细胞色素c1序列的19个氨基酸疏水区的突变阻止了线粒体膜间空间的分类。

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Most mitochondrial proteins destined for the intermembrane space (IMS) carry in their presequence information for localization to the IMS in addition to information for their import. By selecting for mutants in the yeast Saccharomyces cerevisiae that mislocalize an IMS-targeted fusion protein, we identified mutations in the IMS sorting signal of the cytochrome c1 protein. Amino acid substitutions or deletions in a stretch of 19 hydrophobic amino acids of the cytochrome c1 presequence resulted in accumulation of the intermediate form of the cytochrome c1 protein in the matrix. In some cases, the accumulated intermediate appeared to be slowly exported from the matrix, across the inner membrane to the IMS. Our results support the hypothesis that the cytochrome c1 precursor is normally imported completely into the matrix and then exported to the IMS.

机译：除用于导入的信息外，大多数预定进入膜间空间（IMS）的线粒体蛋白还包含其先序列信息以定位到IMS。通过选择酿酒酵母中突变定位于IMS的融合蛋白的突变体，我们在细胞色素c1蛋白的IMS分类信号中鉴定了突变。在细胞色素c1序列的19个疏水氨基酸中，氨基酸取代或缺失导致基质中细胞色素c1蛋白质的中间形式积累。在某些情况下，累积的中间体似乎从基质缓慢地穿过内膜输出到IMS。我们的结果支持以下假设：通常将细胞色素c1前体完全进口到基质中，然后出口到IMS。

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《Molecular and Cellular Biology》 |1992年第10期|共10页
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R E Jensen; S Schmidt; R J Mark;
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中图分类细胞生物学;
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1. Mutations in the Membrane Anchor of Yeast Cytochrome c1 Compensate for the Absence of Oxa1p and Generate Carbonate-Extractable Forms of Cytochrome c1 [J] . Claire Lemaire, Geneviève Dujardin, Nathalie Bonnefoy, Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation . 1998,第2期

机译：酵母细胞色素c1的膜锚中的突变补偿了Oxa1p的缺失，并产生了可从碳酸盐提取的形式的细胞色素c1形式。
2. Functional and mutational characterization of human MIA40 acting during import into the mitochondrial intermembrane space. [J] . Hofmann S, Rothbauer U, Muhlenbein N, Journal of Molecular Biology . 2005,第3期

机译：人类MIA40在导入线粒体膜间空间的过程中的功能和突变特征。
3. Primary structure requirements for correct sorting of the yeast mitochondrial protein ADH III to the yeast mitochondrial matrix space. [J] . D Pilgrim, E T Young Molecular and Cellular Biology . 1987,第1期

机译：正确分类酵母线粒体蛋白ADH III到酵母线粒体基质空间的基本结构要求。
4. REDUCTION POTENTIAL SHIFTS DUE TO ACTIVE SITE AND SURFACE MUTATIONS OF YEAST CYTOCHROME C PEROXIDASE [C] . C. M. DiCarlo, L. B. Vitello, J. E. Erman Electrochemical Society(ECS) Meeting;Symposium on Organic and Biological Electrochemistry General Oral and Poster Session; 20070506-11;20070506-10; Chicago,IL(US);Chicago,IL(US) . 2007

机译：由于酵母细胞色素C过氧化物酶的活性位点和表面突变，降低了潜在的迁移
5. Investigation of mitochondrial protein degradation using mutationally altered forms of yeast cytochrome c. [D] . Chen, Xi. 2003

机译：使用酵母细胞色素c的突变改变形式研究线粒体蛋白质降解
6. Mutations in a 19-amino-acid hydrophobic region of the yeast cytochrome c1 presequence prevent sorting to the mitochondrial intermembrane space. [O] . R E Jensen, S Schmidt, R J Mark 1992

机译：酵母细胞色素c1序列的19个氨基酸疏水区的突变可防止线粒体膜间空间的分选。
7. Mutations in a 19-amino-acid hydrophobic region of the yeast cytochrome c1 presequence prevent sorting to the mitochondrial intermembrane space. [O] . Jensen, R E, Schmidt, S, Mark, R J 1992

机译：酵母细胞色素c1序列的19个氨基酸疏水区的突变可防止线粒体膜间空间的分选。

Mutations in a 19-amino-acid hydrophobic region of the yeast cytochrome c1 presequence prevent sorting to the mitochondrial intermembrane space.

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