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首页> 外文期刊>Molecular and Cellular Biology >Both LyF-1 and an Ets protein interact with a critical promoter element in the murine terminal transferase gene.
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Both LyF-1 and an Ets protein interact with a critical promoter element in the murine terminal transferase gene.

机译：LyF-1和Ets蛋白都与鼠端转移酶基因中的关键启动子元件相互作用。

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Terminal deoxynucleotidyltransferase (TdT) is a template-independent DNA polymerase that is expressed transiently during the earliest stages of B- and T-cell ontogeny. Previously, we characterized the promoter for the murine TdT gene and identified a novel DNA-binding protein, called LyF-1, that interacts with a DNA sequence element found to be critical for transcriptional activity in lymphoid cell lines. Here, we present a more detailed analysis of this 30-bp control element, called the TdT D' element, which is centered approximately 60 bp upstream of the transcription start site. We found that both the murine and human D' elements are recognized by multiple proteins, including LyF-1 and at least two Ets family proteins, Ets-1 and Fli-1. Additional protein-DNA interactions were identified through studies using unfractionated nuclear extracts, in which the D' element was apparently incorporated into a multiprotein complex, possibly containing an Ets protein as a core component. By analyzing a series of substitution mutations, two adjacent binding sites for LyF-1 were identified in the murine D' element, with the Ets protein binding site closely coinciding with the proximal, lower-affinity LyF-1 site. Transient transfection analysis with these mutations revealed that only a 10-bp region, containing precisely the Ets and proximal LyF-1 binding sites, was needed for D' activity. These results suggest an important role for an Ets family protein in the expression of the TdT gene. The role of LyF-1 is less clear; it might act in conjunction with the Ets protein bound at the D' element or it might be unnecessary for D' activity.
机译：末端脱氧核苷酸转移酶（TdT）是一种独立于模板的DNA聚合酶，在B细胞和T细胞发育的最早阶段瞬时表达。以前，我们对鼠TdT基因的启动子进行了表征，并鉴定了一种称为LyF-1的新型DNA结合蛋白，该蛋白与被认为对淋巴样细胞系转录活性至关重要的DNA序列元件相互作用。在这里，我们对这个30 bp的控制元件（称为TdT D'元件）进行了更详细的分析，该元件位于转录起始位点上游约60 bp处。我们发现，鼠类和人类D'元素均被多种蛋白识别，包括LyF-1和至少两种Ets家族蛋白Ets-1和Fli-1。通过使用未分级的核提取物进行的研究确定了其他蛋白质与DNA的相互作用，其中D'元素显然掺入了多蛋白质复合物中，可能含有Ets蛋白作为核心成分。通过分析一系列取代突变，在鼠D'元件中确定了两个相邻的LyF-1结合位点，而Ets蛋白结合位点与近端，较低亲和力的LyF-1位点重合。这些突变的瞬时转染分析表明，D'活性仅需要一个10 bp的区域，即精确地包含Ets和近端LyF-1结合位点。这些结果表明Ets家族蛋白在TdT基因表达中的重要作用。 LyF-1的作用尚不清楚。它可能与结合在D'元件上的Ets蛋白协同作用，或者对于D'活性可能不是必需的。

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