Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.

C Schneider; K Weisshart; L A Guarino; I Dornreiter; E Fanning

首页> 外文期刊>Molecular and Cellular Biology >Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.

【24h】

Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.

机译：DNA聚合酶α-primase与猿猴病毒40（SV40）T抗原的物种特异性功能相互作用需要SV40起源的DNA。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Physical and functional interactions of simian virus 40 (SV40) and polyomavirus large-T antigens with DNA polymerase alpha-primase were analyzed to elucidate the molecular basis for the species specificity of polymerase alpha-primase in viral DNA replication. SV40 T antigen associated more efficiently with polymerase alpha-primase in crude human extracts than in mouse extracts, while polyomavirus T antigen interacted preferentially with polymerase alpha-primase in mouse extracts. The apparent species specificity of complex formation was not observed when purified polymerase alpha-primases were substituted for the crude extracts. Several functional interactions between T antigen and purified polymerase alpha-primase, including stimulation of primer synthesis and primer elongation on M13 DNA in the presence or absence of the single-stranded DNA binding protein RP-A, also proved to be independent of the species from which polymerase alpha-primase had been purified. However, the human DNA polymerase alpha-primase was specifically required for primosome assembly and primer synthesis on SV40 origin DNA in the presence of T antigen and RP-A.

机译：分析了猿猴病毒40（SV40）和多瘤病毒大T抗原与DNA聚合酶α-引物酶的物理和功能相互作用，以阐明聚合酶α-引物酶在病毒DNA复制中的物种特异性的分子基础。 SV40 T抗原在人的粗提物中与聚合酶α-primase的结合比在小鼠提取物中更有效，而多瘤病毒T抗原优先与小鼠提取物中的聚合酶α-primase相互作用。当用纯化的聚合酶α-primases代替粗提物时，没有观察到复合物形成的明显物种特异性。 T抗原和纯化的聚合酶α-引发酶之间的几种功能相互作用，包括在存在或不存在单链DNA结合蛋白RP-A的情况下，刺激引物合成和M13 DNA上的引物伸长，也被认为与该物种无关已纯化了哪种聚合酶α-引物。但是，在T抗原和RP-A存在的情况下，在SV40来源的DNA上进行primosome组装和引物合成时，特别需要使用人类DNA聚合酶α-primase。

著录项

来源
《Molecular and Cellular Biology》 |1994年第5期|共10页
作者
C Schneider; K Weisshart; L A Guarino; I Dornreiter; E Fanning;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Immunologic selection of simian virus 40 (SV40) T-antigen-negative tumor cells which arise by excision of early SV40 DNA. [J] . P T Mora, C L Parrott, K Baksi, Journal of Virology . 1986,第3期

机译：免疫选择Simian病毒40（SV40）T-抗原阴性肿瘤细胞，由早期SV40 DNA切除产生。
2. Immunologic selection of simian virus 40 (SV40) T-antigen-negative tumor cells which arise by excision of early SV40 DNA. [J] . P T Mora, C L Parrott, K Baksi, Journal of Virology . 1986,第3期

机译：免疫选择Simian病毒40（SV40）T-抗原阴性肿瘤细胞，由早期SV40 DNA切除产生。
3. Immunologic selection of simian virus 40 (SV40) T-antigen-negative tumor cells which arise by excision of early SV40 DNA. [J] . P T Mora, C L Parrott, K Baksi, Journal of Virology . 1986,第3期

机译：免疫选择Simian病毒40（SV40）T-抗原阴性肿瘤细胞，由早期SV40 DNA切除产生。
4. Computerized segmentation algorithm with personalized atlases of murine MRIs in a SV40 Large T-antigen mouse mammary cancer model [C] . Adam R. Sibley, Erica Markiewicz, Devkumar Mustafi, Conference on biomedical applications in molecular, structural, and functional imaging . 2016

机译：SV40大型T抗原小鼠乳腺癌模型中带有鼠MRI个性化图谱的计算机分割算法。
5. Interactions of CPI drugs with the SV40 DNA replication origin and characterization of the porphyrin-quadruplex DNA complexes. [D] . Han, Xiaoguang. 1998

机译：CPI药物与SV40 DNA复制起点的相互作用以及卟啉-四链体DNA复合物的表征。
6. Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA. [O] . C Schneider, K Weisshart, L A Guarino, 1994

机译：DNA聚合酶α-primase与猿猴病毒40（SV40）T抗原的物种特异性功能相互作用需要SV40起源的DNA。
7. Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA. [O] . Schneider, C, Weisshart, K, Guarino, L A, 1994

机译：DNA聚合酶α-primase与猿猴病毒40（SV40）T抗原的物种特异性功能相互作用需要SV40起源的DNA。
8. Laser Irradiation of SV40 DNA. [R] . Johnson-Thompson, M., Halpern, J. B., Jackson, W. M., 1982

机译：激光照射sV40 DNa。

Species-specific functional interactions of DNA polymerase alpha-primase with simian virus 40 (SV40) T antigen require SV40 origin DNA.

摘要

著录项

相似文献

相关主题

期刊订阅