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首页> 外文期刊>Molecular and Cellular Biology >Regulation of Drosophila yolk protein genes by an ovary-specific GATA factor.
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Regulation of Drosophila yolk protein genes by an ovary-specific GATA factor.

机译：果蝇卵黄蛋白基因通过卵巢特异性GATA因子的调控。

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摘要

The divergently transcribed yolk protein genes (Yp1 and Yp2) of Drosophila melanogaster are expressed only in adult females, in fat body tissue and in ovarian follicle cells. Using an in vitro transcription assay, we have identified a single 12-bp DNA element that activates transcription from the promoters of both Yp genes. In vivo, this regulatory element is tissue specific: it activates transcription of Yp1 and Yp2 reporter genes in follicle cells but has no detectable effect in fat body or other tissues. The sequence of the element consists of two recognition sites for the GATA family of transcription factors. We show that among the Drosophila genes known to encode GATA factors, only dGATAb is expressed in ovaries. The single transcript that we detect in ovaries is alternatively spliced or initiated to produce an ovary-specific isoform of the protein. Bacterially expressed dGATAb binds to the 12-bp element; a similar binding activity is also present in the Kc0 nuclear extracts used for in vitro transcription assays. These in vitro and in vivo results lead us to propose that dGATAb makes several developmentally regulated products, one of which is a follicle cell-specific protein activating transcription of Yp1 and Yp2 from a known regulatory element.
机译：果蝇的卵黄蛋白基因的转录转录（Yp1和Yp2）仅在成年雌性，脂肪体组织和卵泡细胞中表达。使用体外转录测定法，我们已经鉴定出了一个12 bp DNA元件，该元件可激活两个Yp基因启动子的转录。在体内，这种调节元件是组织特异性的：它激活卵泡细胞中Yp1和Yp2报告基因的转录，但在脂肪体或其他组织中没有可检测的作用。该元件的序列由GATA转录因子家族的两个识别位点组成。我们显示果蝇基因已知编码GATA因子中，只有dGATAb在卵巢中表达。我们在卵巢中检测到的单个转录物被剪接或引发，以产生该蛋白的卵巢特异性同工型。细菌表达的dGATAb与12 bp的元素结合;在用于体外转录测定的Kc0核提取物中也存在类似的结合活性。这些体外和体内的结果使我们提出，dGATAb可以制造几种发育受调控的产物，其中之一是卵泡细胞特异性蛋白，可激活Yp1和Yp2从已知调控元件的转录。

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