Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro.

E Chu; T Takechi; K L Jones; D M Voeller; S M Copur; G F Maley; F Maley; S Segal; C J Allegra

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Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro.

机译：胸苷酸合酶在人结肠癌细胞和体外与c-myc RNA结合。

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Using an immunoprecipitation-reverse transcription-PCR technique, we characterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS protein and the mRNA of the nuclear oncogene c-myc. TS protein is complexed in intact cells with the C-terminal coding region of c-myc mRNA that includes nucleotide positions 1625 to 1790. RNA electrophoretic gel mobility shift assays confirm a specific interaction between TS protein and c-myc mRNA and provide additional evidence that the C-terminal coding region represents an important cis-acting regulatory element. Further evidence demonstrates that the in vitro translational efficiency of c-myc mRNA is inhibited as a result of its direct interaction with TS protein. In addition, the presence of exogenous c-myc mRNA specifically relieves the inhibitory effects of TS protein on TS mRNA translation.

机译：使用免疫沉淀-逆转录-PCR技术，我们在培养的人结肠癌细胞中鉴定了胸苷酸合酶（TS）核糖核蛋白复合物，该复合物由TS蛋白和核癌基因c-myc的mRNA组成。 TS蛋白在完整细胞中与c-myc mRNA的C端编码区（包括1625至1790年核苷酸位置）复合。RNA电泳凝胶迁移率移动分析证实了TS蛋白与c-myc mRNA之间的特异性相互作用，并提供了进一步的证据C端编码区代表重要的顺式调节元件。进一步的证据表明，由于c-myc mRNA与TS蛋白的直接相互作用，其体外翻译效率受到抑制。另外，外源性c-myc mRNA的存在特异性地减轻了TS蛋白对TS mRNA翻译的抑制作用。

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《Molecular and Cellular Biology》 |1995年第1期|共7页
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E Chu; T Takechi; K L Jones; D M Voeller; S M Copur; G F Maley; F Maley; S Segal; C J Allegra;
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中图分类细胞生物学;
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1. The presence of three repeats in the 5' UTR region of thymidylate synthase (TS) is associated with increased TS mRNA expression in cultured human cancer cell lines in vitro. [J] . Hammad H, Sarkar M, Gupta N, Oncology reports . 2012,第1期

机译：胸苷酸合酶（TS）的5'UTR区域中三个重复的存在与体外培养的人类癌细胞系中TS mRNA表达的增加有关。
2. Induction of resistance to the multitargeted antifolate Pemetrexed (ALIMTA) in WiDr human colon cancer cells is associated with thymidylate synthase overexpression. [J] . Sigmond J, Backus HH, Wouters D, Biochemical Pharmacology . 2003,第3期

机译：WiDr人结肠癌细胞中对多靶点抗叶酸Pemetrexed（ALIMTA）的抗性诱导与胸苷酸合酶的过表达有关。
3. Effect of 2'-O-methyl antisense ORNs on expression of thymidylate synthase in human colon cancer RKO cells. [J] . Schmitz JC, Yu D, Agrawal S, Nucleic Acids Research . 2001,第2期

机译：2'-O-甲基反义ORNs对人结肠癌RKO细胞胸苷酸合酶表达的影响。
4. Identification of SRL Binding Receptors on Human Colon Cancer Cells Using Microfluidic-Based LC System With an Advanced Q-TOF MS [C] . Ravindra Gudihal, Sachin M. Eligar, Srikanth Barkeer, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：用高级Q-TOF MS使用基于微流体的LC系统对人结肠癌细胞的SRL结合受体的鉴定
5. The role of nuclear factor-erythroid-2-related factor 2 in sensitivity to thymidylate synthase inhibitors in colon cancer cells. [D] . Clinton, Sarah Ashley. 2016

机译：核因子-类胡萝卜素-2-相关因子2在结肠癌细胞对胸苷酸合酶抑制剂敏感性中的作用。
6. Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro. [O] . E Chu, T Takechi, K L Jones, 1995

机译：胸苷酸合酶在人结肠癌细胞和体外与c-myc RNA结合。
7. Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro. [O] . Chu, E, Takechi, T, Jones, K L, 1995

机译：胸苷酸合酶在人结肠癌细胞和体外与c-myc RNA结合。
8. Retroviral Transfer of Human Mutated Thymidylate Synthase Gene into Hematopoietic Stem Cells for Protection from High-Dose Fluoropyrimidine Toxicity. [R] . Takebe, N., Gallo, R. 2001

机译：将人突变的胸苷酸合酶基因逆转录病毒转移到造血干细胞中以防止大剂量氟嘧啶毒性。

Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro.

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