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首页> 外文期刊>Molecular and Cellular Biology >The cap and the 3' splice site similarly affect polyadenylation efficiency.
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The cap and the 3' splice site similarly affect polyadenylation efficiency.

机译：帽和3'剪接位点类似地影响聚腺苷酸化效率。

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The 5' cap of a mammalian pre-mRNA has been shown to interact with splicing components at the adjacent 5' splice site for processing of the first exon and the removal of the first intron (E. Izaurralde, J. Lewis, C. McGuigan, M. Jankowska, E. Darzynkiewicz, and I.W. Mattaj, Cell 78:657-668, 1994). Likewise, it has been shown that processing of the last exon and removal of the last intron involve interaction between splicing components at the 3' splice site and the polyadenylation complex at the polyadenylation signal (M. Niwa, S. D. Rose, and S.M. Berget, Genes Dev. 4:1552-1559, 1990; M. Niwa and S. M. Berget, Genes Dev. 5:2086-2095, 1991). These findings suggest that the cap provides a function in first exon processing which is similar to the function of the 3' splice site at last exon processing. To determine whether caps and 3' splice sites function similarly, we compared the effects of the cap and the 3' splice site on the in vitro utilization of the simian virus 40 late polyadenylation signal. We show that the presence of a m7GpppG cap, but not a cap analog, can positively affect the efficiency of polyadenylation of a polyadenylation-only substrate. Cap analogs do not stimulate polyadenylation because they fail to bind titratable cap-binding factors. The failure of cap analogs to stimulate polyadenylation can be overcome if a 3' splice site is present upstream of the polyadenylation signal. These data indicate that factors interacting with the cap or the 3' splice site function similarly to affect polyadenylation signal, along with m7GpppG cap, is inhibitory to polyadenylation. This finding suggests that the interaction between the cap-binding complexes and splicing components at the 5' splice site may form a complex which is inhibitory to further processing if splicing of an adjacent intron is not achieved.
机译：已显示哺乳动物pre-mRNA的5'帽与相邻5'剪接位点处的剪接组件相互作用，以处理第一个外显子和去除第一个内含子（E. Izaurralde，J. Lewis，C. McGuigan ，M。Jankowska，E.Darzynkiewicz和IW Mattaj，Cell 78：657-668，1994）。同样，已显示最后一个外显子的加工和最后一个内含子的去除涉及3'剪接位点处的剪接组分与多聚腺苷酸化信号处的聚腺苷酸复合物之间的相互作用（M. Niwa，SD Rose和SM Berget，Genes Dev.4：1552-1559，1990; M.Niwa和SM Berget，Genes Dev.5：2086-2095，1991）。这些发现表明帽在第一外显子加工中提供了功能，类似于在最后的外显子加工中3'剪接位点的功能。为了确定帽和3'剪接位点是否功能相似，我们比较了帽和3'剪接位点对猿猴病毒40晚期聚腺苷酸化信号的体外利用的影响。我们显示m7GpppG帽，但不是帽类似物的存在可以正面影响仅聚腺苷酸底物的聚腺苷酸化效率。帽类似物不刺激聚腺苷酸化，因为它们不能结合可滴定的帽结合因子。如果在聚腺苷酸化信号的上游存在3'剪接位点，则可以克服帽类似物刺激聚腺苷酸化的失败。这些数据表明，与帽或3'剪接位点相互作用的因子与m7GpppG帽一样，也具有影响聚腺苷酸化信号的功能，可抑制聚腺苷酸化。该发现表明，帽结合结合复合物和5'剪接位点处的剪接组分之间的相互作用可能形成复合物，如果未实现相邻内含子的剪接，则该复合物会抑制进一步的加工。

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