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首页> 外文期刊>Molecular and Cellular Biology >Hsp60 Is Targeted to a Cryptic Mitochondrion-Derived Organelle (“Crypton”) in the Microaerophilic Protozoan Parasite Entamoeba histolytica
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Hsp60 Is Targeted to a Cryptic Mitochondrion-Derived Organelle (“Crypton”) in the Microaerophilic Protozoan Parasite Entamoeba histolytica

机译：Hsp60靶向微需氧的原生动物寄生虫组织变形虫中隐匿于线粒体的细胞器（“密码子”）。

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Entamoeba histolytica is a microaerophilic protozoan parasite in which neither mitochondria nor mitochondrion-derived organelles have been previously observed. Recently, a segment of anE. histolytica gene was identified that encoded a protein similar to the mitochondrial 60-kDa heat shock protein (Hsp60 or chaperonin 60), which refolds nuclear-encoded proteins after passage through organellar membranes. The possible function and localization of the amebic Hsp60 were explored here. Like Hsp60 of mitochondria, amebic Hsp60 RNA and protein were both strongly induced by incubating parasites at 42°C. 5′ and 3′ rapid amplifications of cDNA ends were used to obtain the entire E. histolytica hsp60 coding region, which predicted a 536-amino-acid Hsp60. The E. histolytica hsp60 gene protected from heat shockEscherichia coli groEL mutants, demonstrating the chaperonin function of the amebic Hsp60. The E. histolyticaHsp60, which lacked characteristic carboxy-terminal Gly-Met repeats, had a 21-amino-acid amino-terminal, organelle-targeting presequence that was cleaved in vivo. This presequence was necessary to target Hsp60 to one (and occasionally two or three) short, cylindrical organelle(s). In contrast, amebic alcohol dehydrogenase 1 and ferredoxin, which are bacteria-like enzymes, were diffusely distributed throughout the cytosol. We suggest that the Hsp60-associated, mitochondrion-derived organelle identified here be named “crypton,” as its structure was previously hidden and its function is still cryptic.
机译：组织溶Entamoeba 是一种微需氧的原生动物寄生虫，以前既未观察到线粒体，也未观察到线粒体来源的细胞器。最近，一个 E的片段。鉴定出组织蛋白酶基因，该基因编码类似于线粒体60 kDa热休克蛋白（Hsp60或伴侣蛋白60）的蛋白，该蛋白在通过细胞器膜后会重新折叠核编码的蛋白。这里探讨了阿米巴热休克蛋白60的可能功能和定位。像线粒体的Hsp60一样，通过在42°C孵育寄生虫，可以强烈诱导阿米巴Hsp60 RNA和蛋白质。 cDNA末端的5'和3'快速扩增被用于获得整个 E。组织蛋白酶hsp60 编码区，可预测536个氨基酸的Hsp60。 E。保护组织蛋白酶hsp60 基因免受热休克大肠杆菌groEL 突变体的侵袭，证明了阿米巴Hsp60的伴侣蛋白功能。 E。缺乏特征性的羧基末端Gly-Met重复序列的组织蛋白酶Hsp60具有21个氨基酸的氨基末端，细胞器靶向序列，可在体内裂解。要使Hsp60靶向一个（有时是两个或三个）短的圆柱形细胞器，这种先后顺序是必要的。相反，作为细菌样酶的阿米巴醇脱氢酶1和铁氧还蛋白分散在整个细胞质中。我们建议将此处鉴定的与Hsp60相关的线粒体来源的细胞器命名为“密码子”，因为其结构以前是隐藏的，其功能仍是隐秘的。

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