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首页> 外文期刊>Molecular and Cellular Biology >Translational and Structural Requirements of the Early Nodulin Gene enod40, a Short-Open Reading Frame-Containing RNA, for Elicitation of a Cell-Specific Growth Response in the Alfalfa Root Cortex
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Translational and Structural Requirements of the Early Nodulin Gene enod40, a Short-Open Reading Frame-Containing RNA, for Elicitation of a Cell-Specific Growth Response in the Alfalfa Root Cortex

机译：早期Nodulin基因enod40，短开放阅读框RNA的翻译和结构要求，在苜蓿根皮层中引起细胞特异性生长反应。

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摘要

A diversity of mRNAs containing only short open reading frames (sORF-RNAs; encoding less than 30 amino acids) have been shown to be induced in growth and differentiation processes. The early nodulin geneenod40, coding for a 0.7-kb sORF-RNA, is expressed in the nodule primordium developing in the root cortex of leguminous plants after infection by symbiotic bacteria. Ballistic microtargeting of this gene into Medicago roots induced division of cortical cells. Translation of two sORFs (I and II, 13 and 27 amino acids, respectively) present in the conserved 5′ and 3′ regions ofenod40 was required for this biological activity. These sORFs may be translated in roots via a reinitiation mechanism. In vitro translation products starting from the ATG of sORF I were detectable by mutating enod40 to yield peptides larger than 38 amino acids. Deletion of a Medicago truncatula enod40 region between the sORFs, spanning a predicted RNA structure, did not affect their translation but resulted in significantly decreased biological activity. Our data reveal a complex regulation of enod40action, pointing to a role of sORF-encoded peptides and structured RNA signals in developmental processes involving sORF-RNAs.
机译：已显示在生长和分化过程中诱导了仅包含短开放阅读框的多种mRNA（sORF-RNA;编码少于30个氨基酸）。早期结节蛋白基因 enod40 编码0.7 kb sORF-RNA，在被共生细菌感染后在豆科植物根皮中发育的结节原基中表达。对该基因的弹道微靶向作用是 Medicago 根诱导的皮质细胞分裂。该生物活性需要翻译存在于 enod40 的保守5'和3'区域中的两个sORF（分别为I和II，13和27个氨基酸）。这些sORF可以通过重新初始化机制从根目录转换。通过突变 enod40 产生大于38个氨基酸的肽，可以检测到从sORF I的ATG开始的体外翻译产物。 sORF之间的 Medicago truncatula enod40 区域的缺失，跨越一个预测的RNA结构，不会影响其翻译，但会导致生物学活性大大降低。我们的数据揭示了 enod40 作用的复杂调控，指出了sORF编码的肽和结构化RNA信号在涉及sORF-RNA的发育过程中的作用。

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