...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Selective Interactions between Vertebrate Polycomb Homologs and the SUV39H1 Histone Lysine Methyltransferase Suggest that Histone H3-K9 Methylation Contributes to Chromosomal Targeting of Polycomb Group Proteins
【24h】

Selective Interactions between Vertebrate Polycomb Homologs and the SUV39H1 Histone Lysine Methyltransferase Suggest that Histone H3-K9 Methylation Contributes to Chromosomal Targeting of Polycomb Group Proteins

机译：脊椎动物polycomb同源物和SUV39H1组蛋白赖氨酸甲基转移酶之间的选择性相互作用表明组蛋白H3-K9甲基化有助于polycomb组蛋白的染色体靶向。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Polycomb group (PcG) proteins form multimeric chromatin-associated protein complexes that are involved in heritable repression of gene activity. Two distinct human PcG complexes have been characterized. The EED/EZH2 PcG complex utilizes histone deacetylation to repress gene activity. The HPC/HPH PcG complex contains the HPH, RING1, BMI1, and HPC proteins. Here we show that vertebrate Polycomb homologs HPC2 and XPc2, but not M33/MPc1, interact with the histone lysine methyltransferase (HMTase) SUV39H1 both in vitro and in vivo. We further find that overexpression of SUV39H1 induces selective nuclear relocalization of HPC/HPH PcG proteins but not of the EED/EZH2 PcG proteins. This SUV39H1-dependent relocalization concentrates the HPC/HPH PcG proteins to the large pericentromeric heterochromatin domains (1q12) on human chromosome 1. Within these PcG domains we observe increased H3-K9 methylation. Finally, we show that H3-K9 HMTase activity is associated with endogenous HPC2. Our findings suggest a role for the SUV39H1 HMTase and histone H3-K9 methylation in the targeting of human HPC/HPH PcG proteins to modified chromatin structures.
机译：聚梳组（PcG）蛋白形成与染色质相关的多聚体蛋白复合体，这些复合体与基因活性的遗传抑制有关。已经表征了两种不同的人PcG复合物。 EED / EZH2 PcG复合物利用组蛋白去乙酰化来抑制基因活性。 HPC / HPH PcG复合体包含HPH，RING1，BMI1和HPC蛋白。在这里，我们显示脊椎动物的Polycomb同源物HPC2和XPc2，而不是M33 / MPc1，在体外和体内均与组蛋白赖氨酸甲基转移酶（HMTase）SUV39H1相互作用。我们进一步发现SUV39H1的过表达诱导HPC / HPH PcG蛋白的选择性核再定位，而不诱导EED / EZH2 PcG蛋白的选择性核再定位。这种SUV39H1依赖的重新定位将HPC / HPH PcG蛋白集中在人染色体1上的大的着丝粒异染色质域（1q12）上。在这些PcG域中，我们观察到H3-K9甲基化增加。最后，我们显示H3-K9 HMTase活性与内源性HPC2相关。我们的研究结果表明SUV39H1 HMTase和组蛋白H3-K9甲基化在将人HPC / HPH PcG蛋白靶向修饰的染色质结构中具有作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号