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首页> 外文期刊>Molecular and Cellular Biology >Xenopus LSm Proteins Bind U8 snoRNA via an Internal Evolutionarily Conserved Octamer Sequence
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Xenopus LSm Proteins Bind U8 snoRNA via an Internal Evolutionarily Conserved Octamer Sequence

机译：非洲爪蟾LSm蛋白通过内部进化保守的Octamer序列与U8 snoRNA结合

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U8 snoRNA plays a unique role in ribosome biogenesis: it is the only snoRNA essential for maturation of the large ribosomal subunit RNAs, 5.8S and 28S. To learn the mechanisms behind the in vivo role of U8 snoRNA, we have purified to near homogeneity and characterized a set of proteins responsible for the formation of a specific U8 RNA-binding complex. This 75-kDa complex is stable in the absence of added RNA and binds U8 with high specificity, requiring the conserved octamer sequence present in all U8 homologues. At least two proteins in this complex can be cross-linked directly to U8 RNA. We have identified the proteins as Xenopus homologues of the LSm (like Sm) proteins, which were previously reported to be involved in cytoplasmic degradation of mRNA and nuclear stabilization of U6 snRNA. We have identified LSm2, -3, -4, -6, -7, and -8 in our purified complex and found that this complex associates with U8 RNA in vivo. This purified complex can bind U6 snRNA in vitro but does not bind U3 or U14 snoRNA in vitro, demonstrating that the LSm complex specifically recognizes U8 RNA.
机译：U8 snoRNA在核糖体生物发生中发挥独特作用：它是大核糖体亚基5.8S和28S成熟所需的唯一snoRNA。为了了解U8 snoRNA在体内作用背后的机制，我们已经纯化至接近均一并表征了负责形成特定U8 RNA结合复合物的一组蛋白质。这种75 kDa的复合物在不存在添加的RNA的情况下是稳定的，并以高特异性结合U8，需要所有U8同源物中都存在保守的八聚体序列。该复合物中的至少两种蛋白质可以直接与U8 RNA交联。我们已经鉴定出该蛋白质为LSm（如Sm）蛋白质的非洲爪蟾同源物，先前已报道这些蛋白质与mRNA的胞质降解和U6 snRNA的核稳定有关。我们在纯化的复合物中鉴定了LSm2，-3，-4，-6，-7和-8，发现该复合物在体内与U8 RNA缔合。该纯化的复合物可以在体外结合U6 snRNA，但在体外不结合U3或U14 snoRNA，表明LSm复合物特异性识别U8 RNA。

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