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首页> 外文期刊>Molecular and Cellular Biology >The Fission Yeast Nup107-120 Complex Functionally Interacts with the Small GTPase Ran/Spi1 and Is Required for mRNA Export, Nuclear Pore Distribution, and Proper Cell Division
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The Fission Yeast Nup107-120 Complex Functionally Interacts with the Small GTPase Ran/Spi1 and Is Required for mRNA Export, Nuclear Pore Distribution, and Proper Cell Division

机译：裂变酵母Nup107-120复合物在功能上与小GTPase Ran / Spi1相互作用，是mRNA出口，核孔分布和适当细胞分裂所必需的

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We have characterized Schizosaccharomyces pombe open reading frames encoding potential orthologues of constituents of the evolutionarily conserved Saccharomyces cerevisiae Nup84 vertebrate Nup107-160 nuclear pore subcomplex, namely Nup133a, Nup133b, Nup120, Nup107, Nup85, and Seh1. In spite of rather weak sequence conservation, in vivo analyses demonstrated that these S. pombe proteins are localized at the nuclear envelope. Biochemical data confirmed the organization of these nucleoporins within conserved complexes. Although examination of the S. cerevisiae and S. pombe deletion mutants revealed different viability phenotypes, functional studies indicated that the involvement of this complex in nuclear pore distribution and mRNA export has been conserved between these highly divergent yeasts. Unexpectedly, microscopic analyses of some of the S. pombe mutants revealed cell division defects at the restrictive temperature (abnormal septa and mitotic spindles and chromosome missegregation) that were reminiscent of defects occurring in several S. pombe GTPase Ran (RanSp)/Spi1 cycle mutants. Furthermore, deletion of nup120 moderately altered the nuclear location of RanSp/Spi1, whereas overexpression of a nonfunctional RanSp/Spi1-GFP allele was specifically toxic in the Δnup120 and Δnup133b mutant strains, indicating a functional and genetic link between constituents of the S. pombe Nup107-120 complex and of the RanSp/Spi1 pathway.
机译：我们已经表征了 Schizosaccharomyces pombe 开放阅读框，该开放阅读框编码进化保守的 Saccharomyces cerevisiae Nup84脊椎动物Nup107-160核孔亚复合体（即Nup133a，Nup133b，Nup120，Nup107）的成分的潜在直向同源物。 ，Nup85和Seh1。尽管序列保守性较弱，但体内分析表明这些 S。 pombe 蛋白位于核包膜中。生化数据证实了这些核孔蛋白在保守复合物中的组织。虽然 S的检查。酿酒酵母和 S。 pombe 缺失突变体显示出不同的生存表型，功能研究表明，这些复合物在这些高度趋异的酵母菌中参与了核孔分布和mRNA的输出。出乎意料的是，某些 S的微观分析。 pombe 突变体在限制温度下显示细胞分裂缺陷（异常的间隔和有丝分裂纺锤体以及染色体错聚），这使人想起了几个 S中发生的缺陷。 pombe GTPase Ran（Ran Sp ）/ Spi1周期突变体。此外， nup120 的缺失适度地改变了Ran Sp / Spi1的核位置，而过表达非功能性Ran Sp / Spi1-GFP等位基因是在Δ nup120 和Δ nup133b 突变株中具有特定毒性，表明 S的组成之间存在功能和遗传联系。 pombe Nup107-120复合体和Ran Sp / Spi1途径。

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