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首页> 外文期刊>Molecular and Cellular Biology >Transcription Termination Factor reb1p Causes Two Replication Fork Barriers at Its Cognate Sites in Fission Yeast Ribosomal DNA In Vivo
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Transcription Termination Factor reb1p Causes Two Replication Fork Barriers at Its Cognate Sites in Fission Yeast Ribosomal DNA In Vivo

机译：转录终止因子reb1p导致裂变酵母核糖体DNA体内其同源位点的两个复制叉屏障。

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摘要

Polar replication fork barriers (RFBs) near the 3′ end of the rRNA transcriptional unit are a conserved feature of ribosomal DNA (rDNA) replication in eukaryotes. In the mouse, in vivo studies indicate that the cis-acting Sal boxes required for rRNA transcription termination are also involved in replication fork blockage. On the contrary, in the budding yeast Saccharomyces cerevisiae, the rRNA transcription termination factors are not required for RFBs. Here we characterized the rDNA RFBs in the fission yeast Schizosaccharomyces pombe. S. pombe rDNA contains three closely spaced polar replication barriers named RFB1, RFB2, and RFB3 in the 3′ to 5′ order. The transcription termination protein reb1 and its two binding sites, present at the 3′ end of the coding region, were required for fork arrest at RFB2 and RFB3 in vivo. On the other hand, fork arrest at the strongest RFB1 barrier was independent of the above transcription termination factors. Therefore, RFB2 and RFB3 resemble the barriers present in the mouse rDNA, whereas RFB1 is similar to the budding yeast RFBs. These results suggest that during evolution, cis- and trans-acting factors required for rRNA transcription termination became involved in replication fork blockage also. S. pombe is suggested to be a transitional species in which both mechanisms coexist.
机译：rRNA转录单位3'端附近的极性复制叉状屏障（RFB）是真核生物中核糖体DNA（rDNA）复制的保守特征。在小鼠体内研究表明，rRNA转录终止所需的顺式作用Sal盒也参与复制叉的阻断。相反，在萌芽的酿酒酵母中，RFB不需要rRNA转录终止因子。在这里，我们表征裂殖酵母粟酒裂殖酵母中的rDNA RFB。粟酒裂殖酵母rDNA包含3个到5'顺序的三个紧密间隔的极性复制屏障，分别称为RFB1，RFB2和RFB3。转录终止蛋白reb1及其两个结合位点（存在于编码区的3'端）是在体内将叉停在RFB2和RFB3时所必需的。另一方面，在最强的RFB1屏障处的叉停与上述转录终止因子无关。因此，RFB2和RFB3类似于小鼠rDNA中存在的屏障，而RFB1与发芽酵母RFB相似。这些结果表明，在进化过程中，rRNA转录终止所需的 cis -和 trans 作用因子也参与了复制叉的阻断。 S。 Pombe 被认为是两种机制共存的过渡物种。

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