Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae

Donald Prather; Nevan J. Krogan; Andrew Emili; Jack F. Greenblatt; Fred Winston

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Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae

机译：酿酒酵母中保守的转录延伸因子Elf1的鉴定和表征

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In order to identify previously unknown transcription elongation factors, a genetic screen was carried out to identify mutations that cause lethality when combined with mutations in the genes encoding the elongation factors TFIIS and Spt6. This screen identified a mutation in YKL160W, hereafter named ELF1 (elongation factor 1). Further analysis identified synthetic lethality between an elf1Δ mutation and mutations in genes encoding several known elongation factors, including Spt4, Spt5, Spt6, and members of the Paf1 complex. Genome-wide synthetic lethality studies confirmed that elf1Δ specifically interacts with mutations in genes affecting transcription elongation. Chromatin immunoprecipitation experiments show that Elf1 is cotranscriptionally recruited over actively transcribed regions and that this association is partially dependent on Spt4 and Spt6. Analysis of elf1Δ mutants suggests a role for this factor in maintaining proper chromatin structure in regions of active transcription. Finally, purification of Elf1 suggests an association with casein kinase II, previously implicated in roles in transcription. Together, these results suggest an important role for Elf1 in the regulation of transcription elongation.

机译：为了鉴定先前未知的转录延伸因子，进行了遗传筛选以鉴定当与编码延伸因子TFIIS和Spt6的基因中的突变结合时引起致死性的突变。该屏幕确定了YKL160W中的一个突变，此后称为 ELF1 （ el ongation f actor 1）。进一步的分析确定了elf1Δ突变与编码几种已知的延伸因子（包括Spt4，Spt5，Spt6和Paf1复合体成员）的基因之间的突变之间的合成杀伤力。全基因组合成杀伤力研究证实，elf1Δ与影响转录延伸的基因中的突变发生特异性相互作用。染色质的免疫沉淀实验表明，Elf1是在活跃转录的区域上共转录募集的，并且这种关联部分取决于Spt4和Spt6。对elf1Δ突变体的分析表明，该因子在维持活性转录区适当的染色质结构中起作用。最后，Elf1的纯化表明与酪蛋白激酶II有关，后者以前与转录有关。在一起，这些结果表明Elf1在转录延伸的调节中的重要作用。 展开▼

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《Molecular and Cellular Biology》 |2005年第22期|共14页

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Donald Prather; Nevan J. Krogan; Andrew Emili; Jack F. Greenblatt; Fred Winston;
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中图分类细胞生物学;

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1. The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae [J] . Natalia I Reim, James Chuang, Dhawal Jain, Nucleic acids research . 2020,第18期

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2. Uncoupling of the Patterns of Chromatin Association of Different Transcription Elongation Factors by a Histone H3 Mutant in Saccharomyces cerevisiae [J] . Amanda Lloyd, Katie Pratt, Erica Siebrasse, Eukaryotic cell . 2009,第2期

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4. Regulation of Protein Expression by Transcription Factors in Saccharomyces cerevisiae [C] . Gennifer E. Merrihew, Ying Sonia Ting, Michael J. MacCoss ASMS Conference on Mass Spectrometry and Allied Topics . 2014

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5. Characterization of Saccharomyces cerevisiae transcription elongation factor TFIIS by DNA microarray and two-hybrid analysis. [D] . Seidel, Christopher Warren. 2002

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6. Identification and Characterization of Elf1 a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae [O] . Donald Prather, Nevan J. Krogan, Andrew Emili, 2005

机译：酿酒酵母中保守的转录延伸因子Elf1的鉴定和表征

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8. Biochemical and Genetic Characterization of the Transcription Elongation FactorTFIIS from the Yeast Saccharomyces Cerevisiae [R] . Christie, K. R. 1995

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Identification and Characterization of Elf1, a Conserved Transcription Elongation Factor in Saccharomyces cerevisiae

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