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Functional Comparison of the Tup11 and Tup12 Transcriptional Corepressors in Fission Yeast

机译：Tup11和Tup12转录酵母中裂变酵母的功能比较。

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Gene duplication is considered an important evolutionary mechanism. Unlike many characterized species, the fission yeast Schizosaccharomyces pombe contains two paralogous genes, tup11+ and tup12+, that encode transcriptional corepressors similar to the well-characterized budding yeast Tup1 protein. Previous reports have suggested that Tup11 and Tup12 proteins play redundant roles. Consistently, we show that the two Tup proteins can interact together when expressed at normal levels and that each can independently interact with the Ssn6 protein, as seen for Tup1 in budding yeast. However, tup11? and tup12? mutants have different phenotypes on media containing KCl and CaCl2. Consistent with the functional difference between tup11? and tup12? mutants, we identified a number of genes in genome-wide gene expression experiments that are differentially affected by mutations in the tup11+ and tup12+ genes. Many of these genes are differentially derepressed in tup11? mutants and are over-represented in genes that have previously been shown to respond to a range of different stress conditions. Genes specifically derepressed in tup12? mutants require the Ssn6 protein for their repression. As for Tup12, Ssn6 is also required for efficient adaptation to KCl- and CaCl2-mediated stress. We conclude that Tup11 and Tup12 are at least partly functionally diverged and suggest that the Tup12 and Ssn6 proteins have adopted a specific role in regulation of the stress response.
机译：基因复制被认为是重要的进化机制。与许多特征物种不同，裂变酵母 Schizosaccharomyces pombe 包含两个旁系同源基因 tup11 + 和 tup12 + ，其编码与特征明确的出芽酵母Tup1蛋白相似的转录共表达因子。先前的报告表明，Tup11和Tup12蛋白起着多余的作用。一致地，我们显示出两个Tup蛋白在正常水平表达时可以相互作用，并且每个都可以独立地与Ssn6蛋白相互作用，如在发芽酵母中观察到的Tup1。但是， tup11 ？ 和 tup12 ？ 突变体在含有KCl和CaCl 2 的培养基上有不同的表型。与 tup11 ？ 和 tup12 ？ < / sup>突变体，我们在全基因组基因表达实验中发现了许多受 tup11 + 和 tup12 + 基因。这些基因中的许多在 tup11 ？ 突变体中均被差异抑制，并且在以前显示出对一系列不同的压力条件。在 tup12 ？ 突变体中特异抑制的基因需要Ssn6蛋白进行抑制。对于Tup12，还需要Ssn6才能有效适应KCl和CaCl 2 介导的胁迫。我们得出的结论是，Tup11和Tup12在功能上至少有所不同，并暗示Tup12和Ssn6蛋白已在调节应激反应中发挥了特定作用。

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