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首页> 外文期刊>Molecular and Cellular Biology >macroH2A1 Histone Variants Are Depleted on Active Genes but Concentrated on the Inactive X Chromosome
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macroH2A1 Histone Variants Are Depleted on Active Genes but Concentrated on the Inactive X Chromosome

机译：macroH2A1组蛋白变体的活性基因耗尽，但集中于非活性X染色体

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Using a novel thiol affinity chromatography approach to purify macroH2A1-containing chromatin fragments, we examined the distribution of macroH2A1 histone variants in mouse liver chromatin. We found that macroH2A1 was depleted on the transcribed regions of active genes. This depletion was observed on all of the 20 active genes that we probed, with only one site showing a small amount of enrichment. In contrast, macroH2A1 was concentrated on the inactive X chromosome, consistent with our previous immunofluorescence studies. This preferential localization was seen on genes that are active in liver, genes that are inactive in liver, and intergenic regions but was absent from four regions that escape X inactivation. These results support the hypothesis that macroH2As function as transcriptional repressors. Also consistent with this hypothesis is our finding that the heterochromatin protein HP1β copurifies with the macroH2A1-containing chromatin fragments. This study presents the first detailed examination of the distribution of macroH2A1 variants on specific sequences. Our results indicate that macroH2As have complex distribution patterns that are influenced by both local factors and long-range mechanisms.
机译：使用一种新型的硫醇亲和层析方法来纯化含有macroH2A1的染色质片段，我们检查了macroH2A1组蛋白变体在小鼠肝脏染色质中的分布。我们发现macroH2A1耗尽了活跃基因的转录区域。在我们探查的所有20个活性基因中均观察到了这种消耗，只有一个位点显示出少量富集。相反，macroH2A1集中在非活性X染色体上，这与我们之前的免疫荧光研究一致。在肝脏中有活性的基因，肝脏中无活性的基因和基因间区域，但在逃避X灭活的四个区域中没有这种基因，可以看到这种优先定位。这些结果支持了macroH2As充当转录阻遏物的假设。我们的发现也证实了这一假设，即异染色质蛋白HP1β与含有macroH2A1的染色质片段共纯化。这项研究提出了对macroH2A1变体在特定序列上分布的首次详细检查。我们的结果表明，macroH2A具有复杂的分布模式，受本地因素和远程机制的影响。

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