• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Identification of an upstream activation sequence and other cis-acting elements required for transcription of COX6 from Saccharomyces cerevisiae.
【24h】

Identification of an upstream activation sequence and other cis-acting elements required for transcription of COX6 from Saccharomyces cerevisiae.

机译：鉴定从酿酒酵母中转录COX6所需的上游激活序列和其他顺式作用元件。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Transcription of Saccharomyces cerevisiae COX6, the nuclear gene for subunit VI of cytochrome c oxidase, is activated in heme-proficient cells, requires the HAP2 gene, and is subject to glucose repression. In this study, by deletion mutagenesis of the COX6 promoter, we identified two regions that are important for transcription. The first was an upstream activation site, UAS6. It was found to be contained within an 84-base-pair (bp) sequence, between bp -256 and -340 of the COX6 translational initiation codon, and to contain sequences required for activation by heme and HAP2 and for release from glucose repression. When located upstream of a CYC1-lacZ fusion gene, deleted for both of its UASs, this segment functioned as a UAS element. Although UAS6 could promote expression in either orientation, it showed a marked orientation dependence in its response to HAP2 and the carbon source. The second region lay between bp -255 and -91. It contained two of the three major 5' termini of COX6 mRNAs and a putative TATA box. Deletion analysis of this region demonstrated that the putative TATA box is not required for transcription and that this region is separable into two redundant domains.
机译：酿酒酵母COX6的转录是细胞色素c氧化酶亚基VI的核基因，在血红素熟练的细胞中被激活，需要HAP2基因，并受到葡萄糖抑制。在这项研究中，通过删除COX6启动子的诱变，我们确定了两个对转录很重要的区域。第一个是上游激活站点，UAS6。发现它包含在一个84个碱基对（bp）的序列中，在COX6翻译起始密码子的bp -256和-340之间，并且包含被血红素和HAP2激活以及从葡萄糖阻抑释放所需的序列。当位于两个UAS都缺失的CYC1-lacZ融合基因的上游时，该片段充当UAS元件。尽管UAS6可以在任一方向上促进表达，但它对HAP2和碳源的反应显示出明显的方向依赖性。第二个区域位于bp -255和-91之间。它包含COX6 mRNA的三个主要5'末端中的两个和一个假定的TATA盒。该区域的缺失分析表明，不需要转录所需的TATA框，该区域可分为两个冗余结构域。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号