Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.

V J Virta-Pearlman; P H Gunaratne; A C Chinault

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Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.

机译：分析小鼠基因组腺苷脱氨酶区域的复制起始序列。

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A 4-kb HindIII fragment that supported the efficient autonomous replication of plasmid vector pDY-, a replication-defective construct based on Epstein-Barr virus sequences, in human K562 cells was rescued from amplified double-minute chromosomes containing the murine adenosine deaminase locus. Polymerase chain reaction assays of size-fractionated nascent strands demonstrated that replication initiation occurred within the same 1- to 2-kb region of this fragment in autonomously replicating plasmids containing the sequence in either orientation, in double-minute chromosomes, and in the single-copy locus at its normal chromosomal location. The complete sequence of this fragment was determined; it contains a 248-bp polypurine tract and consensus binding site sequences for several putative transcription and replication factors.

机译：从含有鼠腺苷脱氨酶基因座的扩增的两分钟染色体中救出了一个4 kb HindIII片段，该片段支持人类K562细胞中质粒载体pDY-（一种基于爱泼斯坦-巴尔病毒序列的复制缺陷型构建体）的有效自主复制。大小分开的新生链的聚合酶链反应分析表明，复制起始发生在该片段的相同的1至2kb区域内，该复制片段中的自主复制质粒在任一方向，双倍染色体和单染色体中均含有该序列在正常的染色体位置复制基因座。确定了该片段的完整序列。它包含一个248-bp的聚嘌呤束和几个假定的转录和复制因子的共有结合位点序列。

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《Molecular and Cellular Biology》 |1993年第10期|共12页
作者
V J Virta-Pearlman; P H Gunaratne; A C Chinault;
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中图分类细胞生物学;
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4. An enzyme assay mass screening system for adenosine deaminase deficiency from dried blood spots using a DART MS/MS [C] . Hideki Nakajima, Teruhiko Miwa, Kazuhiro Kida, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

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5. Sequence and analysis of approximately 0.5 mega-basepair of the DiGeorge Syndrome Critical Region in human chromosome 22 band q11 and a syntenic mouse BAC [D] . Chen, Feng. 1997

机译：人染色体22带q11和同系小鼠BAC中约0.5兆碱基对的DiGeorge综合征关键区域的序列和分析
6. Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome. [O] . V J Virta-Pearlman, P H Gunaratne, A C Chinault 1993

机译：分析小鼠基因组腺苷脱氨酶区域的复制起始序列。
7. Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome. [O] . Virta-Pearlman, V J, Gunaratne, P H, Chinault, A C 1993

机译：分析小鼠基因组腺苷脱氨酶区域的复制起始序列。
8. Nucleotide Sequence Analysis of Regions of Adenovirus 5 DNA Containing the Origins of DNA Replication [R] . Steenbergh, P. H. 1979

机译：含有DNa复制起源的腺病毒5 DNa区域的核苷酸序列分析

Analysis of a replication initiation sequence from the adenosine deaminase region of the mouse genome.

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