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首页> 外文期刊>Molecular and Cellular Biology >Involvement of a C/EBP-like protein in the acquisition of responsiveness to glucocorticoid hormones during chick neural retina development.
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Involvement of a C/EBP-like protein in the acquisition of responsiveness to glucocorticoid hormones during chick neural retina development.

机译：C / EBP样蛋白参与鸡神经视网膜发育过程中对糖皮质激素的反应性的获得。

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摘要

The glucocorticoid receptor in chicken embryonic neural retina is expressed early in ontogeny, yet the tissue's response to the glucocorticoid hormone, i.e., induction of glutamine synthetase (GS), develops later, only during week 2 of ontogeny. Transient transfection of embryonic day 7 (E7) retinal cells, which are nonresponsive to glucocorticoids, with chimeric plasmids containing the chloramphenicol acetyltransferase reporter gene under the control of glucocorticoid-responsive promoters demonstrated that GR in E7 cells is a functional transactivating factor. We show that the limiting transcription factor that controls the developmental acquisition of responsiveness to glucocorticoids is similar to a CCAAT enhancer-binding protein (C/EBP). This protein recognizes a sequence in the promoter of the chick GS gene, which is required for eliciting the glucocorticoid response. Retinal C/EBP-like protein was not detected in the glucocorticoid-nonresponsive (E7) proliferating glioblasts but was found to be present in the glucocorticoid-responsive (E12) postmitotic cells. Premature expression of C/EBP in the nonresponsive E7 cells by transfection was shown to enhance the developmental acquisition of responsiveness to the glucocorticoid hormone, as deduced from the level of GS inducibility.
机译：鸡胚胎神经视网膜中的糖皮质激素受体在个体发育的早期表达，但是组织对糖皮质激素的反应，即谷氨酰胺合成酶（GS）的诱导，在个体发育的第二周才出现。对含糖皮质激素无反应的胚胎第7天（E7）视网膜细胞的瞬时转染，通过含有在糖皮质激素反应性启动子控制下的氯霉素乙酰转移酶报告基因的嵌合质粒，证明E7细胞中的GR是一种功能性反式激活因子。我们显示，控制对糖皮质激素的反应性发展获得的限制性转录因子类似于CCAAT增强子结合蛋白（C / EBP）。该蛋白识别雏鸡GS基因启动子中的序列，这是引发糖皮质激素反应所必需的。在增殖性非糖皮质激素（E7）的成胶质细胞中未检测到视网膜C / EBP样蛋白，但发现其存在于有糖皮质激素响应（E12）的有丝分裂后细胞中。通过转染，C / EBP在无反应性E7细胞中过早表达可增强对糖皮质激素的反应性，这是从GS诱导水平推论得出的。

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