• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A V(D)J recombinase-inducible B-cell line: role of transcriptional enhancer elements in directing V(D)J recombination.
【24h】

A V(D)J recombinase-inducible B-cell line: role of transcriptional enhancer elements in directing V(D)J recombination.

机译：V（D）J重组酶诱导的B细胞系：转录增强子元件在指导V（D）J重组中的作用。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Rapid analysis of mechanisms that regulate V(D)J recombination has been hampered by the lack of appropriate cell systems that reproduce aspects of normal prelymphocyte physiology in which the recombinase is activated, accessible antigen receptor loci are rearranged, and rearrangement status is fixed by termination of recombinase expression. To generate such a system, we introduced heat shock-inducible V(D)J recombination-activating genes (RAG) 1 and 2 into a recombinationally inert B-cell line. Heat shock treatment of these cells rapidly induced high levels of RAG transcripts and RAG proteins that were accompanied by a parallel induction of V(D)J recombinase activity, strongly suggesting that RAG proteins have a primary role in V(D)J recombination. Within hours after induction, these cells began to rearrange chromosomally integrated V(D)J recombination substrates but only if the substrates contained an active transcriptional enhancer; substrates lacking an enhancer were not efficiently rearranged. Activities necessary to target integrated substrates for rearrangement were provided by two separate lymphoid-specific transcriptional enhancers, as well as an active nonlymphoid enhancer, unequivocally demonstrating that such elements enhance both transcription and V(D)J recombinational accessibility.
机译：缺乏适当的细胞系统来复制正常前淋巴细胞生理学的某些方面，从而阻碍了调节V（D）J重组机制的快速分析，在正常的淋巴细胞生理中，重组酶被激活，可及的抗原受体基因座被重新排列，并且通过终止来固定重排状态重组酶表达。为了生成这样的系统，我们将热休克诱导型V（D）J重组激活基因（RAG）1和2引入了重组惰性B细胞系。这些细胞的热激处理迅速诱导了高水平的RAG转录本和RAG蛋白，并伴随着V（D）J重组酶活性的平行诱导，强烈表明RAG蛋白在V（D）J重组中起主要作用。诱导后数小时内，这些细胞开始重新排列整合了染色体的V（D）J重组底物，但前提是底物含有活性转录增强子。缺少增强剂的底物不能有效地重新排列。由两个单独的淋巴样特异性转录增强子以及一个活性非淋巴增强子提供了靶向整合底物进行重排的必要活性，明确表明这些元素既增强了转录又增强了V（D）J的重组可及性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号