The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.

S Dou; X Zeng; P Cortes; H Erdjument-Bromage; P Tempst; T Honjo; L D Vales

首页> 外文期刊>Molecular and Cellular Biology >The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.

【24h】

The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.

机译：重组信号序列结合蛋白RBP-2N起转录阻遏物的作用。

获取原文

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We have identified a cellular protein, RBP-2N, a presumed recombinase, as a repressor of transcription. Inhibition of transcription by RBP-2N was dependent on its DNA recognition site and was demonstrated in vitro and in vivo. This repression appears to be general, as transcription mediated by SP1 and Gal4/VP16 was inhibited by RBP-2N. The protein was purified to near homogeneity from human cells on the basis of its binding to a site present in the promoter of the adenovirus pIX gene. The DNA recognition sequence is 5'-TGGGAAAGAA, which is markedly different from the recombination signal sequence originally identified as the target site for this protein. The sequence of the purified protein is 97% identical with that published for the mouse RBP-2N protein. The reported homolog in Drosophila is Suppressor of Hairless. RBP-2N binding sites are present in a number of cellular and viral promoters, so RBP-2N may have a general role in transcriptional repression.

机译：我们已经鉴定出一种细胞蛋白RBP-2N（一种推测的重组酶）作为转录的阻遏物。 RBP-2N对转录的抑制取决于其DNA识别位点，并在体外和体内得到证实。这种抑制似乎是普遍的，因为SP1和Gal4 / VP16介导的转录受RBP-2N抑制。根据其与腺病毒pIX基因启动子中存在的位点的结合，从人细胞中纯化出接近同质的蛋白。 DNA识别序列为5'-TGGGAAAGAA，与最初被鉴定为该蛋白质靶位点的重组信号序列明显不同。纯化的蛋白质序列与小鼠RBP-2N蛋白质的序列具有97％的同一性。果蝇中报道的同源物是无毛的抑制物。 RBP-2N结合位点存在于许多细胞和病毒启动子中，因此RBP-2N可能在转录抑制中起一般作用。

著录项

来源
《Molecular and Cellular Biology》 |1994年第5期|共10页
作者
S Dou; X Zeng; P Cortes; H Erdjument-Bromage; P Tempst; T Honjo; L D Vales;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种
中图分类细胞生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Recombination signal sequence-binding protein Jkappa alters mesodermal cell fate decisions by suppressing cardiomyogenesis. [J] . Schroeder T, Fraser ST, Ogawa M, Proceedings of the National Academy of Sciences of the United States of America . 2003,第7期

机译：重组信号序列结合蛋白Jkappa通过抑制心肌发生来改变中胚层细胞的命运决定。
2. A novel cysteine-rich sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex class II genes via a repeated Cys-His domain and functions as a transcriptional repressor. [J] . Z Song, S Krishna, D Thanos, The Journal of Experomental Medicine . 1994,第5期

机译：一种新型的富含半胱氨酸的序列特异性DNA结合蛋白可通过重复的Cys-His结构域与人类主要组织相容性复合体II类基因的保守X-box基序相互作用，并起转录抑制因子的作用。
3. The T alpha 2 nuclear protein binding site from the human T cell receptor alpha enhancer functions as both a T cell-specific transcriptional activator and repressor. [J] . I C Ho, J M Leiden The Journal of Experomental Medicine . 1990,第5期

机译：来自人T细胞受体α增强子的Tα2核蛋白结合位点既充当T细胞特异性转录激活因子，又充当阻遏物。
4. SufR IS A 4Fe-4S PROTEIN THAT FUNCTIONS AS A NEGATIVE TRANSCRIPTIONAL REGULATOR OF THE suf REGULON IN CYANOBACTERIA [C] . Ramakrishnan Balasubramanian, Tao Wang, Gaozhong Shen, International Congress of Photosynthesis . 2005

机译：SUFR是一种4FE-4S蛋白，其用作Cyanobacteria的SUF调节件的阴性转录调节剂
5. Functional organization of the Escherichia coli RecA protein: Alteration of the nucleoside triphosphate catalytic domain, indentification of single-stranded DNA binding site(s), and cleavage of the LexA repressor. [D] . Rehrauer, William Michael. 1996

机译：大肠杆菌RecA蛋白的功能组织：核苷三磷酸催化结构域的改变，单链DNA结合位点的鉴定以及LexA阻遏物的裂解。
6. The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor. [O] . S Dou, X Zeng, P Cortes, 1994

机译：重组信号序列结合蛋白RBP-2N起转录阻遏物的作用。
7. The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor. [O] . Dou, S, Zeng, X, Cortes, P, 1994

机译：重组信号序列结合蛋白RBP-2N起转录阻遏物的作用。

The recombination signal sequence-binding protein RBP-2N functions as a transcriptional repressor.

摘要

著录项

相似文献

相关主题

期刊订阅