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首页> 外文期刊>Molecular and Cellular Biology >Growth factor-induced tyrosine phosphorylation of Hrs, a novel 115-kilodalton protein with a structurally conserved putative zinc finger domain.
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Growth factor-induced tyrosine phosphorylation of Hrs, a novel 115-kilodalton protein with a structurally conserved putative zinc finger domain.

机译：生长因子诱导的Hrs酪氨酸磷酸化，这是一种新型的115千达尔顿蛋白，具有结构保守的假定锌指结构域。

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The activation of growth factor receptor tyrosine kinases leads to tyrosine phosphorylation of many intracellular proteins which are thought to play crucial roles in growth factor signaling pathways. We previously showed that tyrosine phosphorylation of a 115-kDa protein is rapidly induced in cells treated with hepatocyte growth factor. To clarify the structure and possible function of the 115-kDa protein (designated Hrs for hepatocyte growth factor-regulated tyrosine kinase substrate), we purified this protein from B16-F1 mouse melanoma cells by anti-phosphotyrosine immunoaffinity chromatography and determined its partial amino acid sequences. On the basis of the amino acid sequences, we molecularly cloned the cDNA for mouse Hrs. The nucleotide sequence of the cDNA revealed that Hrs is a novel 775-amino-acid protein with a putative zinc finger domain that is structurally conserved in several other proteins. This protein also contained a proline-rich region and a proline- and glutamine-rich region. The expression of Hrs mRNA was detected in all adult mouse tissues tested and also in embryos. To analyze the Hrs cDNA product, we prepared a polyclonal antibody against bacterially expressed Hrs. Using this antibody, we showed by subcellular fractionation that Hrs is localized to the cytoplasm; we also showed that that tyrosine phosphorylation of Hrs is induced in cells treated with epidermal growth factor or platelet-derived growth factor. These results suggest that Hrs plays a unique and important role in the signaling pathway of growth factors.
机译：生长因子受体酪氨酸激酶的激活导致许多细胞内蛋白的酪氨酸磷酸化，这些蛋白被认为在生长因子信号传导途径中起关键作用。我们以前表明，在用肝细胞生长因子处理过的细胞中可快速诱导115 kDa蛋白的酪氨酸磷酸化。为了阐明115 kDa蛋白（肝细胞生长因子调节的酪氨酸激酶底物的指定Hrs）的结构和可能的功能，我们通过抗磷酸酪氨酸免疫亲和色谱法从B16-F1小鼠黑素瘤细胞中纯化了该蛋白，并确定了其部分氨基酸序列。在氨基酸序列的基础上，我们分子克隆了小鼠Hrs的cDNA。 cDNA的核苷酸序列显示，Hrs是一种新型的775个氨基酸蛋白质，具有假定的锌指结构域，该结构在其他几种蛋白质中结构保守。该蛋白质还包含富含脯氨酸的区域以及富含脯氨酸和谷氨酰胺的区域。在所有测试的成年小鼠组织和胚胎中均检测到Hrs mRNA的表达。为了分析Hrs cDNA产物，我们制备了针对细菌表达的Hrs的多克隆抗体。使用该抗体，我们通过亚细胞分级分离显示Hrs定位于细胞质。我们还表明，经表皮生长因子或血小板衍生生长因子处理的细胞可诱导Hrs酪氨酸磷酸化。这些结果表明，Hrs在生长因子的信号传导途径中起着独特而重要的作用。

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