• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Nucleoprotein structure influences the response of the mouse mammary tumor virus promoter to activation of the cyclic AMP signalling pathway.
【24h】

Nucleoprotein structure influences the response of the mouse mammary tumor virus promoter to activation of the cyclic AMP signalling pathway.

机译：核蛋白结构影响小鼠乳腺肿瘤病毒启动子对环状AMP信号通路激活的反应。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Recent studies have provided evidence of crosstalk between steroid receptors and cyclic AMP (cAMP) signalling pathways in the regulation of gene expression. A synergism between intracellular phosphorylation inducers and either glucocorticoids or progestins has been shown to occur during activation of the mouse mammary tumor virus (MMTV) promoter. We have investigated the effect of 8-Br-cAMP and okadaic acid, modulators of cellular kinases and phosphatases, on the hormone-induced activation of the MMTV promoter in two forms: a transiently transfected template with a disorganized, accessible nucleoprotein structure and a stably replicating template with an ordered, inaccessible nucleoprotein structure. Both okadaic acid and 8-Br-cAMP synergize significantly with either glucocorticoids or progestins in activating the transiently transfected MMTV template. In contrast, 8-Br-cAMP, but not okadaic acid, is antagonistic to hormone-induced activation of the stably replicating MMTV template. Nuclear run-on experiments demonstrate that this inhibition is a transcriptional effect on both hormone-induced transcription and basal transcription. Surprisingly, 8-Br-cAMP does not inhibit glucocorticoid-induced changes in restriction enzyme access and nuclear factor 1 binding. However, association of a complex with the TATA box region is inhibited in the presence of 8-Br-cAMP. Thus, cAMP treatment interferes with the initiation process but does not inhibit interaction of the receptor with the template. Since the replicated, ordered MMTV templates and the transfected, disorganized templates show opposite responses to 8-Br-cAMP treatment, we conclude that chromatin structure can influence the response of a promoter to activation of the cAMP signalling pathway.
机译：最近的研究提供了在基因表达调节中类固醇受体和环状AMP（cAMP）信号通路之间串扰的证据。已显示在小鼠乳腺肿瘤病毒（MMTV）启动子激活过程中发生了细胞内磷酸化诱导剂与糖皮质激素或孕激素之间的协同作用。我们已经研究了8-Br-cAMP和冈田酸（细胞激酶和磷酸酶的调节剂）对激素诱导的MMTV启动子活化有两种形式：一种瞬时转染的模板，其杂乱无章，可及的核蛋白结构稳定具有有序的，难以接近的核蛋白结构的复制模板。冈田酸和8-Br-cAMP在激活瞬时转染的MMTV模板中均与糖皮质激素或孕激素显着协同作用。相反，8-Br-cAMP而不是冈田酸对激素诱导的稳定复制MMTV模板的激活具有拮抗作用。核实验表明该抑制作用是对激素诱导的转录和基础转录的转录作用。出人意料的是，8-Br-cAMP不抑制糖皮质激素诱导的限制性内切酶通路和核因子1结合的变化。然而，在8-Br-cAMP的存在下，复合物与TATA盒区的缔合被抑制。因此，cAMP处理会干扰起始过程，但不会抑制受体与模板的相互作用。由于复制的，有序的MMTV模板和转染的，杂乱无章的模板显示出对8-Br-cAMP处理的相反响应，因此我们得出结论，染色质结构可以影响启动子对cAMP信号通路激活的响应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号