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首页> 外文期刊>Molecular and Cellular Biology >Terminal long tandem repeats in chromosomes form Chironomus pallidivittatus.
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Terminal long tandem repeats in chromosomes form Chironomus pallidivittatus.

机译:末端长串联重复在染色体上形成Chironomus pallidivittatus。

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We provide evidence that a chromosome end in the dipteran Chironomus pallidivittatus contains 340-bp tandem repeats reaching the extreme terminus of the chromosome. After adding synthetic oligonucleotide tails to DNA extracted from the microdissected right end of the fourth chromosome, we could demonstrate that the blocks of repeats were tailed at only one end, the chromosome terminus, the interior of the arrays being unavailable for tailing. Using PCR, we furthermore showed that the added tails were connected to 340-bp repeat DNA directly, i.e., without intervening DNA of any other kind. The tailed repeats belong to a subfamily previously known to be the most peripheral one of the different types of 340-bp units. Using plasmid controls, we could also make certain that we did not amplify rare or nonrepresentative DNA termini.
机译:我们提供的证据表明,二倍体Chironomus pallidivittatus中的一条染色体末端包含340 bp的串联重复序列,到达该染色体的最末端。将合成的寡核苷酸尾巴添加到从第四条染色体的显微切割的右端提取的DNA中之后,我们可以证明重复序列的块尾仅在一个末端(染色体末端)被拖尾,阵列内部无法拖尾。使用PCR,我们进一步显示添加的尾巴直接连接到340-bp重复DNA,即不插入任何其他类型的DNA。带尾的重复序列属于先前已知的亚家族,是不同类型的340-bp单元中最外围的一个。使用质粒对照,我们还可以确定我们没有扩增稀有或非代表性的DNA末端。

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