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首页> 外文期刊>Molecular and Cellular Biology >Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae.
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Interaction of a Swi3 homolog with Sth1 provides evidence for a Swi/Snf-related complex with an essential function in Saccharomyces cerevisiae.

机译：Swi3同源物与Sth1的相互作用为酿酒酵母中具有基本功能的Swi / Snf相关复合物提供了证据。

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The Saccharomyces cerevisiae Swi/Snf complex has a role in remodeling chromatin structure to facilitate transcriptional activation. The complex has 11 components, including Swi1/Adr6, Swi2/Snf2, Swi3, Snf5, Snf6, Snf11, Swp73/Snf12, and Tfg3. Mammalian homologs of these proteins have been shown to form multiple Swi/Snf-related complexes. Here we characterize an S. cerevisiae Swi3 homolog (Swh3) and present evidence that it associates in a complex with a Snf2 homolog, Sthl. We identified Swh3 as a protein that interacts with the N terminus of Snf2 in the two-hybrid system. Swh3 and Swi3 are functionally distinct, and overexpression of one does not compensate for loss of the other. Swh3 is essential for viability and does not activate transcription of reporters. The Snf2 sequence that interacts with Swh3 was mapped to a region conserved in Sth1. We show that Swh3 and Sth1 fusion proteins interact in the two-hybrid system and coimmunoprecipitate from yeast cell extracts. We also map interactions between Swh3 and Sth1 and examine the role of a leucine zipper motif in self-association of Swh3. These findings, together with previous analysis of Sth1, indicate that Swh3 and Sth1 are associated in a complex that is functionally distinct from the Swi/Snf complex and essential for viability.
机译：酿酒酵母Swi / Snf复合物在重塑染色质结构以促进转录激活中具有作用。该复合物具有11个组件，包括Swi1 / Adr6，Swi2 / Snf2，Swi3，Snf5，Snf6，Snf11，Swp73 / Snf12和Tfg3。这些蛋白质的哺乳动物同源物已显示形成多种Swi / Snf相关复合物。在这里，我们表征酿酒酵母Swi3同源物（Swh3），并提供证据表明它与Snf2同源物Sthl缔合在一起。我们将Swh3识别为与双杂交系统中Snf2的N末端相互作用的蛋白质。 Swh3和Swi3在功能上是不同的，并且其中一个的过表达不能补偿另一个的丢失。 Swh3对生存力至关重要，并且不会激活报告基因的转录。与Swh3相互作用的Snf2序列被映射到Sth1中保守的区域。我们显示Swh3和Sth1融合蛋白在双杂交系统中相互作用，并从酵母细胞提取物中共免疫沉淀。我们还绘制了Swh3和Sth1之间的相互作用，并检查了亮氨酸拉链基序在Swh3的自我关联中的作用。这些发现以及对Sth1的先前分析表明，Swh3和Sth1在功能上不同于Swi / Snf的复合物中具有相关性，并且对生存能力至关重要。

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