• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >A nucleosome positioned in the distal promoter region activates transcription of the human U6 gene.
【24h】

A nucleosome positioned in the distal promoter region activates transcription of the human U6 gene.

机译：位于远端启动子区域的核小体激活人U6基因的转录。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To investigate the consequences of chromatin reconstitution for transcription of the human U6 gene, we assembled nucleosomes on both plasmids and linear DNA fragments containing the U6 gene. Initial experiments with DNA fragments revealed that U6 sequences located between the distal sequence element (DSE) and the proximal sequence element (PSE) lead to the positioning of a nucleosome partially encompassing these promoter elements. Furthermore, indirect end-labelling analyses of the reconstituted U6 wild-type plasmids showed strong micrococcal nuclease cuts near the DSE and PSE, indicating that a nucleosome is located between these elements. To investigate the influence that nucleosomes exert on U6 transcription, we used two different experimental approaches for chromatin reconstitution, both of which resulted in the observation that transcription of the U6 wild-type gene was enhanced after chromatin assembly. To ensure that the facilitated transcription of the nucleosomal templates is in fact due to a positioned nucleosome, we constructed mutants of the U6 gene in which the sequences between the DSE and PSE were progressively deleted. In contrast to what was observed with the wild-type genes, transcription of these deletion mutants was significantly inhibited when they were packaged into nucleosomes.
机译：为了研究染色质重建对人类U6基因转录的影响，我们在包含U6基因的质粒和线性DNA片段上组装了核小体。 DNA片段的初步实验表明，位于远端序列元件（DSE）和近端序列元件（PSE）之间的U6序列导致部分包围这些启动子元件的核小体的定位。此外，对重组的U6野生型质粒的间接末端标记分析显示，DSE和PSE附近有很强的微球菌核酸酶切割，表明核小体位于这些元件之间。为了研究核小体对U6转录的影响，我们使用了两种不同的染色质重构实验方法，这两种方法均导致观察到染色质组装后U6野生型基因的转录增强。为了确保核小体模板的便利转录实际上是由于定位的核小体，我们构建了U6基因的突变体，其中DSE和PSE之间的序列被逐步删除。与野生型基因观察到的相反，当将这些缺失突变体包装到核小体中时，它们的转录被显着抑制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号