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首页> 外文期刊>Molecular and Cellular Biology >Hepatocyte nuclear factor 3/fork head homolog 11 is expressed in proliferating epithelial and mesenchymal cells of embryonic and adult tissues.
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Hepatocyte nuclear factor 3/fork head homolog 11 is expressed in proliferating epithelial and mesenchymal cells of embryonic and adult tissues.

机译：肝细胞核因子3 /叉头同系物11在胚胎和成年组织的上皮和间充质细胞增殖中表达。

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摘要

The hepatocyte nuclear factor 3alpha (HNF-3alpha) and 3beta proteins have homology in the winged helix/fork head DNA binding domain and regulate cell-specific transcription in hepatocytes and in respiratory and intestinal epithelia. In this study, we describe two novel isoforms of the winged helix transcription factor family, HNF-3/fork head homolog 11A (HFH-11A) and HFH-11B, isolated from the human colon carcinoma HT-29 cell line. We show that these isoforms arise via differential splicing and are expressed in a number of epithelial cell lines derived from tumors (HT-29, Caco-2, HepG2, HeLa, A549, and H441). We demonstrate that differentiation of Caco-2 cells toward the enterocyte lineage results in decreased HFH-11 expression and reciprocal increases in HNF-3alpha and HNF-3beta mRNA levels. In situ hybridization of 16 day postcoitus mouse embryos demonstrates that HFH-11 expression is found in the mesenchymal and epithelial cells of the liver, lung, intestine, renal cortex, and urinary tract. Although HFH-11 exhibits a wide cellular expression pattern in the embryo, its adult expression pattern is restricted to epithelial cells of Lieberkühn's crypts of the intestine, the spermatocytes and spermatids of the testis, and the thymus and colon. HFH-11 expression is absent in adult hepatocytes, but its expression is reactivated in proliferating hepatocytes at 4, 24, and 48 h after partial hepatectomy. Consistent with these findings, we demonstrate that HFH-11 mRNA levels are stimulated by intratracheal administration of keratinocyte growth factor in adult lung and its expression in an adult endothelial cell line is reactivated in response to oxidative stress. These experiments show that the HFH-11 transcription factor is expressed in embryonic mesenchymal and epithelial cells and its expression is reactivated in these adult cell types by proliferative signals or oxidative stress.
机译：肝细胞核因子3alpha（HNF-3alpha）和3beta蛋白在翼状螺旋/叉头DNA结合域中具有同源性，并调节肝细胞以及呼吸道和肠道上皮中的细胞特异性转录。在这项研究中，我们描述了从人结肠癌HT-29细胞系中分离出来的有翅螺旋转录因子家族的两个新型同工型，HNF-3 /叉头同源物11A（HFH-11A）和HFH-11B。我们显示这些同工型通过差异剪接而产生，并在源自肿瘤的许多上皮细胞系中表达（HT-29，Caco-2，HepG2，HeLa，A549和H441）。我们证明，向肠上皮细胞谱系的Caco-2细胞分化导致HFH-11表达下降，而HNF-3alpha和HNF-3beta mRNA水平则相应增加。 16天性交后小鼠胚胎的原位杂交表明，在肝，肺，肠，肾皮质和泌尿道的间充质和上皮细胞中发现了HFH-11表达。尽管HFH-11在胚胎中表现出广泛的细胞表达方式，但其成年表达方式仅限于利伯库恩氏小肠隐窝的上皮细胞，睾丸的精细胞和精细胞以及胸腺和结肠。在成年肝细胞中没有HFH-11表达，但是在部分肝切除术后4、24和48小时，其表达在增殖的肝细胞中被重新激活。与这些发现一致，我们证明气管内施用成年肺中的角质形成细胞生长因子刺激了HFH-11 mRNA的水平，并且其在成年内皮细胞系中的表达响应了氧化应激而被重新激活。这些实验表明，HFH-11转录因子在胚胎间质和上皮细胞中表达，并且在这些成年细胞类型中，其表达通过增殖信号或氧化应激而被重新激活。

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