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首页> 外文期刊>Molecular and Cellular Biology >The Properties of a tRNA-Specific Adenosine Deaminase from Drosophila melanogaster Support an Evolutionary Link between Pre-mRNA Editing and tRNA Modification
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The Properties of a tRNA-Specific Adenosine Deaminase from Drosophila melanogaster Support an Evolutionary Link between Pre-mRNA Editing and tRNA Modification

机译：果蝇的tRNA特异性腺苷脱氨酶的特性支持前mRNA编辑和tRNA修饰之间的进化联系。

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摘要

Pre-mRNA editing involving the conversion of adenosine to inosine is mediated by adenosine deaminases that act on RNA (ADAR1 and ADAR2). ADARs contain multiple double-stranded RNA(dsRNA)-binding domains in addition to an adenosine deaminase domain. An adenosine deaminase acting on tRNAs, scTad1p (also known as scADAT1), cloned fromSaccharomyces cerevisiae has a deaminase domain related to the ADARs but lacks dsRNA-binding domains. We have identified a gene homologous to scADAT1 in the region of Drosophila melanogaster Adh chromosome II. Recombinant Drosophila ADAT1 (dADAT1) has been expressed in the yeast Pichia pastorisand purified. The enzyme has no activity on dsRNA substrates but is a tRNA deaminase with specificity for adenosine 37 of insect alanine tRNA. dADAT1 shows greater similarity to vertebrate ADARs than to yeast Tad1p, supporting the hypothesis of a common evolutionary origin for ADARs and ADATs. dAdat1 transcripts are maternally supplied in the egg. Zygotic expression is widespread initially and later concentrates in the central nervous system.
机译：涉及腺苷向肌苷转化的前mRNA编辑是通过作用于RNA（ADAR1和ADAR2）的腺苷脱氨基酶介导的。除腺苷脱氨酶结构域外，ADAR还包含多个双链RNA（dsRNA）结合结构域。从啤酒酵母中克隆的作用于tRNA的腺苷脱氨酶scTad1p（也称为scADAT1）具有与ADAR相关的脱氨酶结构域，但缺少dsRNA结合结构域。我们已经确定了与果蝇果蝇Adh II号染色体区域中与scADAT1同源的基因。重组果蝇 ADAT1（dADAT1）已在酵母 Pichia pastoris 中表达并纯化。该酶对dsRNA底物没有活性，但是是一种tRNA脱氨酶，对昆虫丙氨酸tRNA的腺苷37具有特异性。与酵母Tad1p相比，dADAT1与脊椎动物ADAR的相似性更高，支持了ADAR和ADAT共同进化起源的假说。卵中母体提供了 dAdat1 转录本。合子表达最初很普遍，后来集中在中枢神经系统。

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