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首页> 外文期刊>Molecular and Cellular Biology >Attachment of HeT-A Sequences to Chromosomal Termini in Drosophila melanogaster May Occur by Different Mechanisms
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Attachment of HeT-A Sequences to Chromosomal Termini in Drosophila melanogaster May Occur by Different Mechanisms

机译：HeT-A序列对果蝇的染色体末端的附着可能通过不同的机制发生。

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摘要

Drosophila telomeres contain arrays of the retrotransposonlike elements HeT-A and TART. Their transposition to broken chromosomal termini has been implicated in chromosome healing and telomere elongation. The HeT-Aelement is attached by its 3′ end, which contains the promoter. To monitor the behavior of HeT-A elements, we used the yellow gene with terminal deficiencies consisting of breaks in theyellow promoter region that result in they-null phenotype. Attachment of the HeT-Aelement provides the promoterless yellow gene with a promoter that activates yellow expression in bristles. The frequency of HeT-A transpositions to the yellowterminal deficiency depends on the genotype of the line and varies from 2 × 10?3 to less than 2 × 10?5. Loss of the attached HeT-A due to incomplete replication at the telomere leads to inactivation of yellow expression, which is restored by attachment of a new HeT-A element upstream of yellow. New HeT-A additions occur at a frequency of about 1.2 × 10?3. Short DNA attachments are generated by gene conversion using the homologous telomeric sequences as templates. Longer DNA attachments are generated either by conventional transposition of an HeT-A element to the chromosomal terminus or by recombination between the 3′ terminus of telomeric HeT-A elements and the receding end ofHeT-A attached to the yellow gene.
机译：果蝇端粒包含逆转录转座子样元件 HeT-A 和 TART 的阵列。它们转位至断裂的染色体末端与染色体修复和端粒延长有关。 HeT-A 元件通过其3'末端连接，该末端包含启动子。为了监测 HeT-A 元件的行为，我们使用了带有黄色末端末端缺陷的黄色基因，该缺陷由黄色启动子区域的断裂导致 y -空表型。 HeT-A 元件的附着为无启动子的 yellow 基因提供了一个启动子，该启动子激活了刚毛中的 yellow 表达。 HeT-A 易位到黄色末端缺陷的频率取决于品系的基因型，范围从2×10 ？3 到更低大于2×10 ？5 。由于端粒复制不完全导致附着的 HeT-A 丢失，导致 yellow 表达失活，通过附着新的 HeT-A < / em>黄色上游的元素。新的 HeT-A 加入频率约为1.2×10 ？3 。通过使用同源端粒序列作为模板的基因转化，可以产生短的DNA附件。通过将 HeT-A 元件常规转座至染色体末端，或通过端粒 HeT-A 元件的3'末端与后座的重组产生更长的DNA附着黄色基因的 HeT-A 末端。

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