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首页> 外文期刊>Molecular and Cellular Biology >Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.
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Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.

机译：7号染色体可抑制无限增殖的永生化人类成纤维细胞系KMST-6和SUSM-1的无限分裂。

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摘要

Using nontumorigenic immortalized human cell lines KMST-6 (KMST) and SUSM-1 (SUSM), we attempted to identify the chromosome that carries a putative senescence-related gene(s). These cell lines are the only ones that have been established independently from normal human diploid fibroblasts following in vitro mutagenesis. We first examined restriction fragment length polymorphisms on each chromosome of these immortalized cell lines and their parental cell lines and found specific chromosomal alterations common to these cell lines (a loss of heterozygosity in KMST and a deletion in SUSM) on the long arm of chromosome 7. In addition to these, we also found that introduction of chromosome 7 into these cell lines by means of microcell fusion resulted in the cessation of cell division, giving rise to cells resembling cells in senescence. Introduction of other chromosomes, such as chromosomes 1 and 11, on which losses of heterozygosity were also detected in one of the cell lines (KMST), to either KMST or SUSM cells or of chromosome 7 to several tumor-derived cell lines had no effect on their division potential. These results strongly suggest that a gene(s) affecting limited-division potential or senescence of normal human fibroblasts is located on chromosome 7, probably at the long arm of the chromosome, representing the first case in which a specific chromosome reverses the immortal phenotype of otherwise normal human cell lines.
机译：我们使用非致瘤性永生化人类细胞系KMST-6（KMST）和SUSM-1（SUSM），试图鉴定携带推定衰老相关基因的染色体。这些细胞系是在体外诱变后与正常人二倍体成纤维细胞独立建立的唯一细胞系。我们首先检查了这些永生化细胞系及其亲本细胞系的每个染色体上的限制性片段长度多态性，并在第7号染色体的长臂上发现了这些细胞系共有的特定染色体改变（KMST杂合性缺失和SUSM缺失）。除了这些，我们还发现通过微细胞融合将7号染色体引入这些细胞系导致细胞分裂的停止，从而导致衰老的细胞类似于细胞。将其他染色体（例如1号和11号染色体）导入KMST或SUSM细胞，或将7号染色体导入其中的一种肿瘤细胞株，这些染色体在其中一种细胞系（KMST）中也检测到杂合性丧失他们的分裂潜力。这些结果有力地表明，影响正常人成纤维细胞有限分裂潜力或衰老的一个基因位于第7号染色体上，可能位于该染色体的长臂上，这代表了第一种情况，即特定染色体逆转了其永生表型。否则是正常的人类细胞系。

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