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首页> 外文期刊>Molecular and Cellular Biology >A monocistronic transcript for a trypanosome variant surface glycoprotein.
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A monocistronic transcript for a trypanosome variant surface glycoprotein.

机译：锥虫变体表面糖蛋白的单顺反子转录物。

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Many protein-encoding genes of African trypanosomes are transcribed as large polycistronic pre-mRNAs that are processed into individual mRNAs containing a 5' spliced leader and 3' poly(A). The 45- to 60-kb pre-mRNAs encoding some variant surface glycoproteins (VSGs) contain as many as eight unrelated coding regions. Here we identify the promoter for a metacyclic VSG gene that is expressed without duplication in a bloodstream trypanosome clone. This 70-bp promoter is located 2 kb upstream of the telomere-linked VSG gene and directs the synthesis of a monocistronic VSG pre-mRNA lacking the 5' spliced leader. Its sequence only slightly resembles those of other known trypanosome promoters, but it does cross-hybridize with several related sequences elsewhere in the genome. These results suggest that a new class of trypanosome promoters has been found, whose function is to initiate monocistronic transcription of those VSG genes normally expressed during the metacyclic stage.
机译：非洲锥虫的许多蛋白质编码基因被转录为大型多顺反子前mRNA，被加工成包含5'拼接前导序列和3'poly（A）的单个mRNA。编码某些变异表面糖蛋白（VSG）的45至60 KB前mRNA包含多达八个不相关的编码区。在这里，我们确定了一个在血液锥虫克隆中无重复表达的元环VSG基因的启动子。这个70 bp的启动子位于端粒连接的VSG基因上游2 kb，并指导缺乏5'拼接前导序列的单顺反子VSG pre-mRNA的合成。它的序列仅与其他已知锥虫启动子的序列略相似，但确实与基因组中其他位置的几个相关序列交叉杂交。这些结果表明，已经发现了一类新的锥虫启动子，其功能是启动那些在元周期阶段正常表达的VSG基因的单顺反子转录。

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